Rabbit Crk II Polyclonal Antibody | anti-CRK antibody

Crk II, phosphorylated (Tyr221)

Cat. Num. AAA614715

• Gene Names: CRK; CRKII
• Reactivity: Hamster, Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Crk II; Polyclonal Antibody; phosphorylated (Tyr221); Anti -Crk II; anti-CRK antibody

• Host: Rabbit
• Reactivity: Hamster, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of CrkII only when phosphorylated at tyrosine 221 at 42kD. Crossreactivity
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-CRK antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000; Note: for Western Blots incubate membrane with diluted antibody in 5% BSA, 1X TBS, and 0.1% Tween-20 at 4ºC with gentle shaking overnight.
• Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr221 of human CrkII.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of K562 cell lysates treated with calf intestinal phosphatase (CIP), using MBS614715.)

Related Product Information for anti-CRK antibody

CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases (1). The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk (2). The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mamalian CrkII proteins (3). Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase (4), IGF-I receptor (5) and EGF receptor (6). Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex (3).

Product Categories/Family for anti-CRK antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 219555
• NCBI GeneID: 1398
• UniProt Accession #: P46108; Q96GA9; Q96HJ0; A8MWE8; B0LPE8; D3DTH6
• Molecular Weight: 33,831 Da
• NCBI Official Full Name: CRK-II
• NCBI Official Synonym Full Names: v-crk sarcoma virus CT10 oncogene homolog (avian)
• NCBI Official Symbol: CRK
• NCBI Official Synonym Symbols: CRKII
• NCBI Protein Information: adapter molecule crk; p38; OTTHUMP00000115366; OTTHUMP00000198330; proto-oncogene c-Crk
• UniProt Protein Name: Adapter molecule crk
• Protein Family: CDPK-related protein kinase
• UniProt Gene Name: CRK
• UniProt Entry Name: CRK_HUMAN

NCBI Description

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq]

Uniprot Description

CRK: an adaptor protein with an SH2-SH3-SH3 domain structure. Recruits cytoplasmic proteins through SH2-phospho-tyrosine interaction. Phosphorylated by Abl, IGF-IR and EGFR. Phosphorylation induces a change in intramolecular folding and SH2 interactions, causing its rapid dissociation from the tyrosine kinase complex.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold; Oncoprotein

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus; actin cytoskeleton

Molecular Function: protein phosphorylated amino acid binding; protein binding; ephrin receptor binding; SH3/SH2 adaptor activity; SH2 domain binding

Biological Process: regulation of transcription from RNA polymerase II promoter; regulation of Rac protein signal transduction; platelet activation; nerve growth factor receptor signaling pathway; activation of MAPKK activity; regulation of actin cytoskeleton organization and biogenesis; insulin receptor signaling pathway; ephrin receptor signaling pathway; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway