Rabbit ABCA7 Polyclonal Antibody | anti-ABCA7 antibody

ABCA7 Polyclonal Antibody

Cat. Num. AAA3006386

• Gene Names: ABCA7; AD9; ABCX; ABCA-SSN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ABCA7; Polyclonal Antibody; ABCA7 Polyclonal Antibody; ATP-binding cassette sub-family A member 7; ABCA-SSN; Autoantigen SS-N; Macrophage ABC transporter; anti-ABCA7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ABCA7 polyclonal antibody detects endogenous levels of ABCA7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 2146

• Applicable Applications for anti-ABCA7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human ABCA7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ABCA7 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ABCA7 pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ABCA7 pAb in paraffin-embedded human liver carcinoma tissue at 1:50,showing membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ABCA7 pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-ABCA7 antibody

ATP-binding cassette (ABC) transporters are an evolutionarily conserved family of widely-expressed proteins that use ATP hydrolysis to catalyze the transport of various molecules across extracellular and intracellular membranes. Eukaryotic ABC transporters are largely responsible for trafficking hydrophobic compounds either within the cell as part of a metabolic process, outside the cell for transport to other organs, or for secretion from the body. The cholesterol-responsive transporter, ABCA7, maps to human chromosome 19 and mouse chromosome 10 and has been reported as a candidate regulator of ceramide transport in epidermal lipid reorganization. High expression levels of ABCA7 have been reported in myelolymphatic tissues, reticuloendothelial cells, peripheral leukocytes, thymus, spleen and bone marrow. This expression pattern of the two alternatively-spliced isoforms also indicates an involvement in lipid homeostasis in cells of the immune system, though the complete role of ABCA7 is not yet known. Full-length type I ABCA7 has shown plasma membrane localization, while the type II splicing variant has shown expression predominantly in the endoplasmic reticulum.

NCBI and Uniprot Product Information

• NCBI GI #: 150417984
• NCBI GeneID: 10347
• NCBI Accession #: NP_061985
• NCBI GenBank Nucleotide #: NP_061985.2
• UniProt Accession #: Q8IZY2
• Molecular Weight: ~ 234kDa
• NCBI Official Full Name: phospholipid-transporting ATPase ABCA7
• NCBI Official Synonym Full Names: ATP binding cassette subfamily A member 7
• NCBI Official Symbol: ABCA7
• NCBI Official Synonym Symbols: AD9; ABCX; ABCA-SSN
• NCBI Protein Information: phospholipid-transporting ATPase ABCA7
• UniProt Protein Name: ATP-binding cassette sub-family A member 7
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA7
• UniProt Entry Name: ABCA7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA7: Plays a role in phagocytosis by macrophages of apoptotic cells. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocytes differentiation. Belongs to the ABC transporter superfamily. ABCA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; Golgi apparatus; cell surface; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; ATP-binding cassette (ABC) transporter complex; phagocytic cup

Molecular Function: apolipoprotein A-I receptor activity; phospholipid transporter activity; transporter activity; ATPase activity; ATP binding

Biological Process: phospholipid efflux; positive regulation of ATPase activity; positive regulation of phagocytosis; negative regulation of amyloid precursor protein biosynthetic process; cholesterol efflux; peptide cross-linking; phospholipid scrambling; negative regulation of ATPase activity; transmembrane transport; phagocytosis; memory