Human ATP Binding Cassette Transporter A7 (ABCA7) ELISA Kit | ABCA7 elisa kit

Human ATP Binding Cassette Transporter A7 (ABCA7) ELISA Kit

Cat. Num. AAA2707637

• Gene Names: ABCA7; AD9; ABCX; ABCA-SSN
• Reactivity: Human
• Synonyms: ATP Binding Cassette Transporter A7 (ABCA7); Human ATP Binding Cassette Transporter A7 (ABCA7) ELISA Kit; ABC-A7; ABCA-SSN; ABCX; Autoantigen SS-N; Macrophage ABC transporter; ABCA7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter A7 (ABCA7).; No significant cross-reactivity or interference between ATP Binding Cassette Transporter A7 (ABCA7) and analogues was observed.
• Sequence Length: 2146

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.117ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter A7 (ABCA7) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter A7 (ABCA7) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCA7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter A7 (ABCA7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter A7 (ABCA7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter A7 (ABCA7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter A7 (ABCA7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 150417984
• NCBI GeneID: 10347
• NCBI Accession #: NP_061985
• NCBI GenBank Nucleotide #: NP_061985.2
• UniProt Accession #: Q8IZY2
• NCBI Official Full Name: phospholipid-transporting ATPase ABCA7
• NCBI Official Synonym Full Names: ATP binding cassette subfamily A member 7
• NCBI Official Symbol: ABCA7
• NCBI Official Synonym Symbols: AD9; ABCX; ABCA-SSN
• NCBI Protein Information: phospholipid-transporting ATPase ABCA7
• UniProt Protein Name: ATP-binding cassette sub-family A member 7
• Protein Family: ABC transporter A family
• UniProt Gene Name: ABCA7
• UniProt Entry Name: ABCA7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCA7: Plays a role in phagocytosis by macrophages of apoptotic cells. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocytes differentiation. Belongs to the ABC transporter superfamily. ABCA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; Golgi apparatus; cell surface; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; ATP-binding cassette (ABC) transporter complex; phagocytic cup

Molecular Function: apolipoprotein A-I receptor activity; phospholipid transporter activity; transporter activity; ATPase activity; ATP binding

Biological Process: phospholipid efflux; positive regulation of ATPase activity; positive regulation of phagocytosis; negative regulation of amyloid precursor protein biosynthetic process; cholesterol efflux; peptide cross-linking; phospholipid scrambling; negative regulation of ATPase activity; transmembrane transport; phagocytosis; memory