Western Blot (WB)
(Western Blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.)
Rabbit PP2Calpha Polyclonal Antibody | anti-PP2Calpha antibody
PP2Calpha Polyclonal Antibody
ICC: 1:100-1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.)
Western Blot (WB)
(Western Blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.)
Immunocytochemistry (ICC)
(ICC staining PP2C alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PP2C alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.
Protein type: Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16
Chromosomal Location of Human Ortholog: 14q23.1
Cellular Component: nucleoplasm; neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol
Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity
Biological Process: transcription initiation from RNA polymerase II promoter; N-terminal protein myristoylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; Wnt receptor signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of NF-kappaB import into nucleus; negative regulation of I-kappaB kinase/NF-kappaB cascade; protein amino acid dephosphorylation; dephosphorylation; transforming growth factor beta receptor signaling pathway; insulin receptor signaling pathway; gene expression; cell cycle arrest; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of Wnt receptor signaling pathway
Research Articles on PP2Calpha
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Product Notes
The PP2Calpha ppm1a (Catalog #AAA3013283) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP2Calpha Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PP2Calpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 ICC: 1:100-1:500 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PP2Calpha ppm1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2Calpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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