Rabbit A1Up Polyclonal Antibody | anti-A1Up antibody

A1Up Antibody

Cat. Num. AAA9602045

• Gene Names: UBQLN4; A1U; A1Up; UBIN; CIP75; C1orf6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: A1Up; Polyclonal Antibody; A1Up Antibody; 28S ribosomal protein S7; A1U; AI663987; Ataxin 1 interacting ubiquitin like protein; Ataxin 1 ubiquitin like interacting protein A1U; Ataxin 1 ubiquitin like interacting protein; Ataxin-1 ubiquitin-like-interacting protein A1U; bMRP 27a; bMRP27a; C1orf6; CIP75; Connexin43 interacting protein of 75 kDa; MRP S7; RGD1308273; S7mt; UBIN; Ubiquilin 4; Ubiquilin-4; UBQL4_HUMAN; UBQLN4; anti-A1Up antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A1Up antibody detects endogenous levels of total A1Up
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 581

• Applicable Applications for anti-A1Up antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000
• Immunogen: A synthesized peptide derived from human A1Up
• Subcellular Location: Nucleus. Cytoplasm. Endoplasmic Reticulum. May associate with the endoplasmic reticulum.
• Tissue Specificity: Highly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of A1Up using COLO205 whole cell lysates)

Western Blot (WB)

(Western blot analysis of extracts from mouse brain, using A1Up Antibody. Lane 1 was treated with the blocking peptide.)

Related Product Information for anti-A1Up antibody

Function: Plays a role in the regulation of protein degradation via the ubiquitin-proteasome system (UPS). Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:15280365). Plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1 (By similarity). Acts as an adapter protein that recruits UBQLN1 to the autophagy machinery. Mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3 (MAP1LC3A/B/C) and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:23459205).
Subunit Structure: Homooligomer (PubMed:15280365). Binds signal sequences of proteins that are targeted to the endoplasmic reticulum. Interacts (via UBA domain) with GJA1 (not ubiquitinated) and with ubiquitin; both compete for the same binding site. Interacts (via UBA domain) with ubiquitin and with polyubiquitin chains. Interacts with PSMD2 (By similarity). Interacts (via ubiquitin-like domain) with PSMD4, a regulatory subunit of the 26S proteasome. Interacts with ATXN1/SCA1. Interaction with ATXN1 inhibits polyubiquitination of UBQLN4 and interferes with PSMD4 binding. Interacts with HERPUD1. Interacts (via ubiquitin-like domain) with UBQLN1 (via UBA domain). Interacts (via STI1 1 and 2 domains) with MAP1LC3A/B/C. Interacts with UBQLN2.
Post-translational Modifications: Ubiquitinated; this does not lead to proteasomal degradation. May undergo both 'Lys-48'-and 'Lys-63'-linked polyubiquitination.

NCBI and Uniprot Product Information

• NCBI GI #: 747811802
• NCBI GeneID: 56893
• NCBI Accession #: NP_001291271.1
• NCBI GenBank Nucleotide #: NM_001304342.1
• UniProt Accession #: Q9NRR5; Q5VYA0; Q5VYA1; Q9BR98; Q9UHX4; A6ND44; B2RAY7
• Molecular Weight: Observed: 54 kDa; Predicted: 64 kDa
• NCBI Official Full Name: ubiquilin-4 isoform 2
• NCBI Official Synonym Full Names: ubiquilin 4
• NCBI Official Symbol: UBQLN4
• NCBI Official Synonym Symbols: A1U; A1Up; UBIN; CIP75; C1orf6
• NCBI Protein Information: ubiquilin-4
• UniProt Protein Name: Ubiquilin-4
• UniProt Gene Name: UBQLN4
• UniProt Synonym Gene Names: C1orf6; CIP75; UBIN; A1Up; CIP75

Uniprot Description

Plays a role in the regulation of protein degradation via the ubiquitin-proteasome system (UPS). Mediates the proteasomal targeting of misfolded or accumulated proteins for degradation by binding (via UBA domain) to their polyubiquitin chains and by interacting (via ubiquitin-like domain) with the subunits of the proteasome (PubMed:15280365). Plays a role in the regulation of the proteasomal degradation of non-ubiquitinated GJA1 (). Acts as an adapter protein that recruits UBQLN1 to the autophagy machinery. Mediates the association of UBQLN1 with autophagosomes and the autophagy-related protein LC3 (MAP1LC3A/B/C) and may assist in the maturation of autophagosomes to autolysosomes by mediating autophagosome-lysosome fusion (PubMed:23459205).