Sheep anti-Mouse 5'-Nucleotidase Polyclonal Antibody | anti-NT5E antibody

5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT)

Cat. Num. AAA617992

• Gene Names: NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: 5'-Nucleotidase; Polyclonal Antibody; 5'-Nucleotidase (CD73; Ecto-5'nucleotidase; 5'-NT); Anti -5'-Nucleotidase (CD73; anti-NT5E antibody

• Host: Sheep
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse CD73.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-NT5E antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Direct ELISA, Western Blot. and Flow Cytometry.; Dilution: Direct ELISA: 0.5-1.0ug/ml.Detection limit for rmCD73 is; ~0.3ng/well.; Western Blot: 0.1-0.2ug/ml. Detection limit for rmCD73 is ~1ng/lane under non-reducing and reducing conditions.; Flow Cytometry (Murine splenocytes): 25ug/ml. Add 100ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not excedding 200ul.
• Immunogen: CHO cell-derived rmCD73. (aa 29-549)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NT5E antibody

CD73 (Ecto-5'nucleotidase, 5 '-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

Product Categories/Family for anti-NT5E antibody

Antibodies; Abs to Enzymes; Nucleotidase

NCBI and Uniprot Product Information

• NCBI GI #: 23897
• NCBI GeneID: 4907
• UniProt Accession #: P21589; O75520; Q5W116; Q53Z63; Q5JRQ2; Q6NZX3; Q96B60
• Molecular Weight: 63,368 Da
• NCBI Official Full Name: 5'-nucleotidase
• NCBI Official Synonym Full Names: 5'-nucleotidase, ecto (CD73)
• NCBI Official Symbol: NT5E
• NCBI Official Synonym Symbols: NT; eN; NT5; NTE; eNT; CD73; E5NT
• NCBI Protein Information: 5'-nucleotidase; 5'-NT; OTTHUMP00000016808; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• Protein Family: 5'-nucleotidase
• UniProt Gene Name: NT5E
• UniProt Synonym Gene Names: NT5; NTE
• UniProt Entry Name: 5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Phosphatase (non-protein); Membrane protein, GPI anchor; EC 3.1.3.5; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries