CD73 Recombinant Protein | NT5E recombinant protein

CD73 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120571

• Gene Names: NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD73; CD73 Protein; Human; Recombinant (hFc Tag); Human CD73/NT5E Protein (Fc Tag); CALJA Protein; E5NT Protein; eN Protein; eNT Protein; NT Protein; NT5 Protein; NTE Protein; 5'-nucleotidase; ecto (CD73); NT5E recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys547 (T376A)

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Trp 27
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human NT5E (NP_002517.1, with natural variant Thr 376 Ala)(Met1-Lys547) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NT5E recombinant protein

Background: 5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.

Product Categories/Family for NT5E recombinant protein

Phosphatases and Regulators

References

Resta R. et al., 1997, Cell Signal. 9 (2): 131-9. Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90. Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55. Grunewald JK. et al., 2010, J Inflamm. 7 (1): 10.

NCBI and Uniprot Product Information

• NCBI GI #: 4505467
• NCBI GeneID: 4907
• NCBI Accession #: NP_002517.1
• NCBI GenBank Nucleotide #: NM_002526.3
• UniProt Accession #: P21589; O75520; Q5W116; B3KQI8
• Molecular Weight: 57,949 Da
• NCBI Official Full Name: 5'-nucleotidase isoform 1 preproprotein
• NCBI Official Synonym Full Names: 5'-nucleotidase, ecto (CD73)
• NCBI Official Symbol: NT5E
• NCBI Official Synonym Symbols: NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
• NCBI Protein Information: 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
• UniProt Protein Name: 5'-nucleotidase
• UniProt Gene Name: NT5E
• UniProt Synonym Gene Names: NT5; NTE; 5'-NT
• UniProt Entry Name: 5NTD_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries