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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human 3',5'-Bisphosphate Nucleotidase 1 Polyclonal Antibody | anti-BPNT1 antibody

Polyclonal Antibody to 3',5'-Bisphosphate Nucleotidase 1 (BPNT1)

Gene Names
BPNT1; PIP; HEL20
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
3'; 5'-Bisphosphate Nucleotidase 1; Polyclonal Antibody; Polyclonal Antibody to 3'; 5'-Bisphosphate Nucleotidase 1 (BPNT1); PIP; BPntase; Bisphosphate 3-Prime-Nucleotidase; Bisphosphate 3'-nucleotidase 1; PAP-inositol 1; 4-phosphatase; anti-BPNT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against BPNT1. It has been selected for its ability to recognize BPNT1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
308
Applicable Applications for anti-BPNT1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
BPNT1 (Val7~Pro308)
Conjugate
No Conjugate
Cross Reactivity
Human
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant BPNT1 (Val7~Pro308) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073651
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin- embedded kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,189 Da
NCBI Official Full Name
3'(2'),5'-bisphosphate nucleotidase 1 isoform 2
NCBI Official Synonym Full Names
3'(2'), 5'-bisphosphate nucleotidase 1
NCBI Official Symbol
BPNT1
NCBI Official Synonym Symbols
PIP; HEL20
NCBI Protein Information
3'(2'),5'-bisphosphate nucleotidase 1
UniProt Protein Name
3'(2'),5'-bisphosphate nucleotidase 1
UniProt Gene Name
BPNT1
UniProt Synonym Gene Names
PIP

NCBI Description

BPNT1, also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq, Jul 2008]

Uniprot Description

BPNT1: Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4- trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins(1)P, Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6. Belongs to the inositol monophosphatase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.7; Energy Metabolism - sulfur; Motility/polarity/chemotaxis; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: cytosol

Molecular Function: 3'(2'),5'-bisphosphate nucleotidase activity; metal ion binding

Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; dephosphorylation; nervous system development; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; phosphatidylinositol phosphorylation

Research Articles on BPNT1

Similar Products

Product Notes

The BPNT1 bpnt1 (Catalog #AAA2033900) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to 3',5'-Bisphosphate Nucleotidase 1 (BPNT1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 3',5'-Bisphosphate Nucleotidase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the BPNT1 bpnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "3',5'-Bisphosphate Nucleotidase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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