Western Blot (WB)
(Western Blot: Sample: Recombinant LGMN, Mouse.)
Rabbit anti-Mouse Legumain Polyclonal Antibody | anti-LGMN antibody
Polyclonal Antibody to Legumain (LGMN)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant LGMN, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LGMN, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Skeletal muscle Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Skeletal muscle Tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis. [provided by RefSeq, Aug 2016]
Uniprot Description
LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.
Protein type: EC 3.4.22.34; Protease
Chromosomal Location of Human Ortholog: 12|12 E
Cellular Component: apical part of cell; late endosome; lysosome
Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; hydrolase activity; peptidase activity
Biological Process: negative regulation of multicellular organism growth; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; response to acidic pH; vacuolar protein processing
Research Articles on LGMN
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Product Notes
The LGMN lgmn (Catalog #AAA2028483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Legumain (LGMN) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Legumain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the LGMN lgmn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Legumain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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