DNA-directed RNA polymerase III subunit RPC3 Recombinant Protein | RPC3 recombinant protein

Recombinant Human DNA-directed RNA polymerase III subunit RPC3

Cat. Num. AAA9421412

• Gene Names: POLR3C; RPC3; RPC62
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: DNA-directed RNA polymerase III subunit RPC3; Recombinant Human DNA-directed RNA polymerase III subunit RPC3; DNA-directed RNA polymerase III subunit CRNA polymerase III 62 kDa subunit; RPC62; RPC3 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 1-534aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for RPC3 recombinant protein

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other mbers of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as tplate for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 742800211
• NCBI GeneID: 10623
• NCBI Accession #: NP_001290385.1
• NCBI GenBank Nucleotide #: NM_001303456.1
• UniProt Accession #: Q9BUI4; O15317; Q9Y3R6
• Molecular Weight: 76.6kD
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC3 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase III subunit C
• NCBI Official Symbol: POLR3C
• NCBI Official Synonym Symbols: RPC3; RPC62
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC3
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC3
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR3C
• UniProt Synonym Gene Names: RNA polymerase III subunit C3; RPC62

Uniprot Description

POLR3C: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB- DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.

Protein type: EC 2.7.7.6; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex; Transferase

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: cytosol; DNA-directed RNA polymerase III complex; nucleoplasm

Molecular Function: DNA-directed RNA polymerase activity; protein binding

Biological Process: positive regulation of innate immune response; positive regulation of interferon type I production; positive regulation of interferon-beta production; regulation of transcription from RNA polymerase III promoter