Rabbit anti-Human RPC3 Polyclonal Antibody | anti-RPC3 antibody

RPC3 Antibody

Cat. Num. AAA9605461

• Gene Names: POLR3C; RPC3; RPC62
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RPC3; Polyclonal Antibody; RPC3 Antibody; DNA directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C; DNA-directed RNA polymerase III subunit rpc3; POLR3C; Polymerase (RNA) III (DNA directed) polypeptide C (62kD); RNA polymerase C subunit 3; RNA polymerase III 62 kDa subunit; RNA polymerase III C62 subunit; RNA polymerase III subunit C3; RPC3_HUMAN; RPC62; anti-RPC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RPC3 antibody detects endogenous levels of total RPC3
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 547

• Applicable Applications for anti-RPC3 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Nucleus
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Similarity: Pig (80%), Bovine (91%), Horse (82%), Sheep (82%), Rabbit (82%), Dog (82%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunofluorescence (IF)

(MBS9605461 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from K562 cells using RPC3 antibody.)

Related Product Information for anti-RPC3 antibody

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner (PubMed:21358628).
Subunit Structure: Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex (PubMed:9171375, PubMed:12391170). Directly interacts with POLR3G and POLR3GL (PubMed:24107381, PubMed:21358628, PubMed:26394183). Directly interacts with POLR3F/RPC39 (PubMed:26394183). Interacts with GTF3C4 (PubMed:10523658).
Similarity: Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.

NCBI and Uniprot Product Information

• NCBI GI #: 742800211
• NCBI GeneID: 10623
• NCBI Accession #: NP_001290385.1
• NCBI GenBank Nucleotide #: NM_001303456.1
• UniProt Accession #: Q9BUI4; O15317; Q9Y3R6
• Molecular Weight: Observed: 60 kD; Predicted: 61 kDa
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC3 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase III subunit C
• NCBI Official Symbol: POLR3C
• NCBI Official Synonym Symbols: RPC3; RPC62
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC3
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC3
• UniProt Gene Name: POLR3C
• UniProt Synonym Gene Names: RNA polymerase III subunit C3; RPC62

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner (PubMed:21358628).