Mouse NGF-Receptor (p75) /CD271 Monoclonal Antibody | anti-NGFR antibody

NGF-Receptor (p75) /CD271 (Soft Tissue Tumor Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439581

• Gene Names: NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• Reactivity: Human, Monkey, Baboon, Cat, Rabbit, Ferret.; Does not react with Mouse and Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: NGF-Receptor (p75) /CD271; Monoclonal Antibody; NGF-Receptor (p75) /CD271 (Soft Tissue Tumor Marker) Mouse Monoclonal Antibody; CD271; Gp80-LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Nerve growth factor receptor (NGFR); TNFR superfamily member 16; p75 ICD; p75 Neurotrophin receptor; Tumor necrosis factor receptor superfamily member 16 (TNFRSF16); anti-NGFR antibody

• Host: Mouse
• Reactivity: Human, Monkey, Baboon, Cat, Rabbit, Ferret.; Does not react with Mouse and Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM299
• Specificity: It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 427

• Applicable Applications for anti-NGFR antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cell Surface and Cytoplasmic
• Immunogen: NGFR from A875 melanoma cells
• Hu-Chromosome Location: 17q21-22
• Positive Control: Neuronal axons, Schwann cells, and perineural cells of peripheral nerves, or tumors of nerve sheath differentiation e.g. Schwannoma, Neurofibroma. Soma and axons of sensory neurons, and ganglionic satellite cells. Melanomas.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR Monoclonal Antibody (SPM299).)

References

Marano N, et. al. Journal of Neurochemistry, 1987, 48:225-32.

NCBI and Uniprot Product Information

• NCBI GI #: 4505393
• NCBI GeneID: 4804
• NCBI Accession #: NP_002498.1
• NCBI GenBank Nucleotide #: NM_002507.3
• UniProt Accession #: P08138; B2R961; B4E096
• Molecular Weight: 75kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: NGFR
• NCBI Official Synonym Symbols: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: NGFR
• UniProt Synonym Gene Names: TNFRSF16; NGF receptor
• UniProt Entry Name: TNR16_HUMAN

NCBI Description

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

Uniprot Description

NGFR: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with LINGO1.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: cell surface; cytosol; endosome; extracellular region; integral to plasma membrane; neuron projection; nucleoplasm; nucleus; plasma membrane

Molecular Function: nerve growth factor binding; protein binding; Rab GTPase binding; receptor activity; signal transducer activity; transmembrane receptor activity; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: axon guidance; caspase activation; circadian regulation of gene expression; glucose homeostasis; immune response; inflammatory response; intracellular protein transport; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of caspase activity; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of axonogenesis; positive regulation of MAPKKK cascade; regulation of apoptosis; regulation of caspase activity; regulation of cell proliferation; response to lipopolysaccharide