Chicken Purine nucleoside phosphorylase ELISA Kit | PNP elisa kit

Chicken Purine nucleoside phosphorylase (PNP) ELISA Kit

Cat. Num. AAA2603721

• Gene Names: PNP; NP; PUNP; PRO1837
• Reactivity: Chicken
• Synonyms: Purine nucleoside phosphorylase; Chicken Purine nucleoside phosphorylase (PNP) ELISA Kit; PNP elisa kit

• Reactivity: Chicken
• Specificity: No cross-reaction with other factors.
• Sequence Length: 289

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PNP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken PNP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 157168362
• NCBI GeneID: 4860
• NCBI Accession #: NP_000261.2
• NCBI GenBank Nucleotide #: NM_000270.3
• Molecular Weight: 32,118 Da
• NCBI Official Full Name: purine nucleoside phosphorylase
• NCBI Official Synonym Full Names: purine nucleoside phosphorylase
• NCBI Official Symbol: PNP
• NCBI Official Synonym Symbols: NP; PUNP; PRO1837
• NCBI Protein Information: purine nucleoside phosphorylase
• UniProt Protein Name: Purine nucleoside phosphorylase
• Protein Family: Purine nucleoside phosphorylase
• UniProt Gene Name: PNP
• UniProt Synonym Gene Names: NP; PNP
• UniProt Entry Name: PNPH_HUMAN

NCBI Description

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children.

Protein type: EC 2.4.2.1; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - purine; Transferase; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: cytoplasm; cytoskeleton; cytosol; intracellular; nucleus

Molecular Function: drug binding; nucleoside binding; phosphate binding; purine binding; purine-nucleoside phosphorylase activity

Biological Process: immune response; inosine catabolic process; nicotinamide riboside catabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; positive regulation of alpha-beta T cell differentiation; positive regulation of T cell proliferation; purine base metabolic process; purine nucleotide catabolic process; purine salvage; response to drug

Disease: Purine Nucleoside Phosphorylase Deficiency