Fish Phospho-Nuclear Factor Kappa B ELISA Kit | PNFKB elisa kit

Fish Phospho-Nuclear Factor Kappa B ELISA Kit

Cat. Num. AAA15059

• Gene Names: PPP1R14A; CPI17; CPI-17; PPP1INL
• Reactivity: Fish
• Synonyms: Phospho-Nuclear Factor Kappa B; Fish Phospho-Nuclear Factor Kappa B ELISA Kit; PNFKB elisa kit

• Reactivity: Fish
• Specificity: No significant cross-reactivity or interference between Fish PNFKB and analogues was observed.
• Sequence Length: 120

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml - 2000 pg/ml.
• Sensitivity: 10 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PNFKB concentrations in Fish serum, plasma and other body fluids. Using Purified Fish PNFKB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PNFKB and PNFKB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PNFKB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PNFKB elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PNFKB concentrations in Fish serum, plasma and other body fluids. Using Purified Fish PNFKB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PNFKB and PNFKB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PNFKB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 345441807
• NCBI GeneID: 94274
• NCBI Accession #: NP_001230876.1
• NCBI GenBank Nucleotide #: NM_001243947.1
• UniProt Accession #: Q96A00; Q7Z4X7; Q96S54
• Molecular Weight: 16,693 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 14A isoform 2
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 14A
• NCBI Official Symbol: PPP1R14A
• NCBI Official Synonym Symbols: CPI17; CPI-17; PPP1INL
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 14A; 17-KDa protein; PKC-potentiated inhibitory protein of PP1; 17 kDa PKC-potentiated inhibitory protein of PP1; 17-kDa PKC-potentiated inhibitory protein of PP1; protein kinase C-potentiated inhibitor protein of 17 kDa
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 14A
• UniProt Gene Name: PPP1R14A
• UniProt Synonym Gene Names: CPI17; PPP1INL; CPI-17
• UniProt Entry Name: PP14A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A.

Protein type: Inhibitor; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm

Molecular Function: protein phosphatase inhibitor activity

Biological Process: regulation of phosphorylation; negative regulation of catalytic activity