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Western Blot (WB) (Western Blot analysis of MED6 in Hela cell using MED6 Rabbit pAb at dilution 1/250)

Rabbit anti-Human, Mouse MED6 Polyclonal Antibody | anti-MED6 antibody

MED6 Rabbit pAb

Gene Names
MED6; ARC33; NY-REN-28
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
MED6; Polyclonal Antibody; MED6 Rabbit pAb; mediator complex subunit 6; ARC33; NY-REN-28; anti-MED6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.6mg/ml (varies by lot)
Applicable Applications for anti-MED6 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC-P: 1:25-1:100
Immunogen
Full length fusion protein
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of MED6 in Hela cell using MED6 Rabbit pAb at dilution 1/250)

Western Blot (WB) (Western Blot analysis of MED6 in Hela cell using MED6 Rabbit pAb at dilution 1/250)

Immunohistochemistry (IHC)

(Immunohistochemistry of MED6 in paraffin-embedded Human liver cancer tissue using MED6 Rabbit pAb at dilution 1/20. Predicted cell location: Nucleus)

Immunohistochemistry (IHC) (Immunohistochemistry of MED6 in paraffin-embedded Human liver cancer tissue using MED6 Rabbit pAb at dilution 1/20. Predicted cell location: Nucleus)
Related Product Information for anti-MED6 antibody
Mediator of RNA polymerase II transcription subunit 6 is one of the subunits of the Mediator complex. It is an enzyme that in humans is encoded by the MED6 gene. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,425 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 6 isoform 4
NCBI Official Synonym Full Names
mediator complex subunit 6
NCBI Official Symbol
MED6
NCBI Official Synonym Symbols
ARC33; NY-REN-28
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 6; renal carcinoma antigen NY-REN-28; activator-recruited cofactor 33 kDa component
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 6
UniProt Gene Name
MED6
UniProt Synonym Gene Names
ARC33; ARC33; hMed6
UniProt Entry Name
MED6_HUMAN

Uniprot Description

MED6: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 6 family.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 14q24.2

Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus

Molecular Function: protein binding; transcription coactivator activity; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression

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Product Notes

The MED6 med6 (Catalog #AAA4760178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MED6 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MED6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC-P: 1:25-1:100. Researchers should empirically determine the suitability of the MED6 med6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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