PLEKHO1 / Pleckstrin Homology Domain-Containing Family O Member 1 Recombinant Protein | PLEKHO1 recombinant protein

Human PLEKHO1 / Pleckstrin Homology Domain-Containing Family O Member 1 Recombinant Protein

• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: PLEKHO1 / Pleckstrin Homology Domain-Containing Family O Member 1; Human PLEKHO1 / Pleckstrin Homology Domain-Containing Family O Member 1 Recombinant Protein; Pleckstrin homology domain-containing family O member 1; PH domain-containing family O member 1; C-Jun-binding protein; JBP; Casein kinase 2-interacting protein 1; CK2-interacting protein 1; Osteoclast maturation-associated gene 120 protein; CKIP1; OC120; HQ0024c; PLEKHO1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 409

• Applicable Applications for PLEKHO1 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Heterodimer or homodimer. Interacts with CK2 and actin capping subunits (capping protein CP-alpha and CP-beta). CKIP1 and CK2 together inhibit the activity of actin capping protein at the barbed ends of actin filaments. Interacts with ATM, IFP35, JUN, JUND, NMI and PI3K. Interacts with AKT1, AKT2 and AKT3 (each isozyme of PKB), PtdIns(3,5)P2, PtdIns(4,5)P2 and PtdIns(3,4,5)P2.
• Entry Function: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 20149626
• NCBI Accession #: NP_057358
• NCBI GenBank Nucleotide #: NP_057358.2
• UniProt Accession #: Q53GL0
• NCBI Official Full Name: pleckstrin homology domain-containing family O member 1 isoform a
• UniProt Protein Name: Pleckstrin homology domain-containing family O member 1
• Protein Family: Pleckstrin homology domain-containing family
• UniProt Gene Name: PLEKHO1
• UniProt Synonym Gene Names: CKIP1; OC120; PH domain-containing family O member 1; JBP; CK2-interacting protein 1; CKIP-1
• UniProt Entry Name: PKHO1_HUMAN

Uniprot Description

PLEKHO1: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT- mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding