Rabbit anti-Human NXF5 Polyclonal Antibody | anti-NXF5 antibody

NXF5, NT (NXF5, TAPL1, Nuclear RNA export factor 5, TAP-like protein 1) (FITC)

Cat. Num. AAA6327362

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NXF5; Polyclonal Antibody; NT (NXF5; TAPL1; Nuclear RNA export factor 5; TAP-like protein 1) (FITC); TAP-like protein 1; anti-NXF5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NXF5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NXF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-80 amino acids from the N-terminal region of human NXF5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NXF5 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NXF5 Antibody detected the NXF5 protein (arrow).)

Related Product Information for anti-NXF5 antibody

This gene is one member of a family of nuclear RNA export factor genes. The encoded protein can bind RNA, and is implicated in mRNA nuclear export. However, this protein has lost several C-terminal protein domains found in other family members that are required for export activity, and may be an evolving pseudogene. Alternatively spliced transcript variants have been described, but most are candidates for nonsense-mediated decay (NMD) and may not express proteins in vivo.

Product Categories/Family for anti-NXF5 antibody

Antibodies; Nuclear Proteins

References

Tarpey, P.S., et al. Nat. Genet. 41(5):535-543(2009). Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004): Frints, S.G., et al. Am. J. Med. Genet. A 119A (3), 367-374 (2003): Jun, L., et al. Curr. Biol. 11(18):1381-1391(2001). Herold, A., et al. Mol. Cell. Biol. 20(23):8996-9008(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 254039592
• NCBI GeneID: 55998
• NCBI Accession #: NP_116564.2
• NCBI GenBank Nucleotide #: NM_032946.2
• UniProt Accession #: Q9H1B4; Q9H1B0; Q9H1B1; Q9H1B2; Q9H1B3; A2RRM0; B1AV82; B1AV83; B1AV84; B1AV85
• Molecular Weight: 45,628 Da
• NCBI Official Full Name: nuclear RNA export factor 5
• NCBI Official Synonym Full Names: nuclear RNA export factor 5
• NCBI Official Symbol: NXF5
• NCBI Protein Information: nuclear RNA export factor 5; TAPL-1; TAP-like protein 1
• UniProt Protein Name: Nuclear RNA export factor 5
• Protein Family: Nuclear RNA export factor
• UniProt Gene Name: NXF5
• UniProt Synonym Gene Names: TAPL1; TAPL-1
• UniProt Entry Name: NXF5_HUMAN

NCBI Description

This gene is one member of a family of nuclear RNA export factor genes. The encoded protein can bind RNA, and is implicated in mRNA nuclear export. However, this protein has lost several C-terminal protein domains found in other family members that are required for export activity, and may be an evolving pseudogene. Alternatively spliced transcript variants have been described, but most are candidates for nonsense-mediated decay (NMD) and may not express proteins in vivo. [provided by RefSeq, Jul 2009]

Uniprot Description

NXF5: Could be involved in the export of mRNA from the nucleus to the cytoplasm. Could also have a role in polarized cytoplasmic transport and localization of mRNA in neurons. A chromosomal aberration involving NXF5 has been observed in one patient with a syndromic form of mental retardation and short stature. Pericentric inversion inv(X)(p21.1;q22) that interrupts NXF5. Belongs to the NXF family. 6 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: mRNA export from nucleus; multicellular organismal development; RNA transport