Phospholipase C Epsilon 1 Recombinant Protein | PLCe1 recombinant protein
Recombinant Phospholipase C Epsilon 1 (PLCe1)
Gene Names
PLCE1; PLCE; PPLC; NPHS3
Applications
SDS-Page, Western Blot
Purity
>90%
Synonyms
Phospholipase C Epsilon 1; Recombinant Phospholipase C Epsilon 1 (PLCe1); PLCE; NPHS3; PPLC; Phosphoinositide phospholipase C-epsilon-1; Pancreas-enriched phospholipase C; 1-phosphatidylinositol 4; 5-bisphosphate phosphodiesterase epsilon-1; PLCe1 recombinant protein
Host
E Coli
Purity/Purification
>90%
Form/Format
Freeze-dried powder
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence Positions
Met1~Gln250
Sequence Length
1994
Applicable Applications for PLCe1 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Human
Tag
N-terminal His and GST Tag
Subcellular Location
Secreted
Isoelectric Point
5.9
Reconstitution
Reconstitute in PBS or others.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Product Categories/Family for PLCe1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 58.1kDa
Accurate Molecular Mass: 58kDa
Accurate Molecular Mass: 58kDa
NCBI Official Full Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 2
NCBI Official Synonym Full Names
phospholipase C epsilon 1
NCBI Official Symbol
PLCE1
NCBI Official Synonym Symbols
PLCE; PPLC; NPHS3
NCBI Protein Information
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
UniProt Protein Name
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Protein Family
UniProt Gene Name
PLCE1
UniProt Synonym Gene Names
KIAA1516; PLCE; PPLC; PLC-epsilon-1
UniProt Entry Name
PLCE1_HUMAN
NCBI Description
This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
Research Articles on PLCe1
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Product Notes
The PLCe1 plce1 (Catalog #AAA2123153) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Met1~Gln250. AAA Biotech's Phospholipase C Epsilon 1 can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the PLCe1 plce1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholipase C Epsilon 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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