Western Blot (WB)
(Western blot analysis of PLCE1 expression in HEK293 cells,The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human PLCE1 Polyclonal Antibody | anti-PLCE1 antibody
PLCE1 Antibody
Western Blot (WB)
(Western blot analysis of PLCE1 expression in HEK293 cells,The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of PLCE1 expression in HEK293 cells,The lane on the left is treated with the antigen-specific peptide.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
Uniprot Description
PLCE1: a widely expressed bifunctional enzyme with phospholipase activity and Ras guanine-exchange factor (RasGEF) activity. Catalyzes the release of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) from 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. Is an effector of heterotrimeric G protein activation. May play a role in cell survival, cell growth, actin organization and T-cell activation. Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs. Interacts with RHOA. Interacts with IQGAP1, HRAS, RAP1A, RAP2A, RAP2B and RRAS. Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Requires calcium for activiation. The Ras-associating domain 1 is degenerate and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane. The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway. Defects in PLCE1 are the cause of nephrotic syndrome type 3 (NPHS3). Nephrotic syndrome, a malfunction of the kidney glomerular filter, leads to proteinuria, hypoalbuminemia, edema. Overexpressed during heart failure. A GWAS study identified an association between esophageal squamous cell carcinoma and a common DNA variant in the PLCE1 gene in East Asians. Two isoforms of the human protein are produced by alternative splicing. Isoform 1 is broadly expressed and only absent in peripheral blood leukocytes. Isoform 2 is specifically expressed in placenta, lung and spleen. Isoform 1 and isoform 2 associate with Golgi membranes.
Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; GAPs; GAPs, Ras; Phospholipase
Chromosomal Location of Human Ortholog: 10q23
Cellular Component: cytoplasm; cytosol; plasma membrane
Molecular Function: enzyme binding; guanyl-nucleotide exchange factor activity; phosphoinositide phospholipase C activity; phospholipase C activity; protein binding; Ras GTPase binding; receptor signaling protein activity
Biological Process: activation of MAPK activity; calcium-mediated signaling; diacylglycerol biosynthetic process; elevation of cytosolic calcium ion concentration; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); glomerulus development; heart development; inositol phosphate metabolic process; inositol phosphate-mediated signaling; phospholipid metabolic process; Ras protein signal transduction; regulation of cell growth; regulation of G-protein coupled receptor protein signaling pathway; regulation of protein kinase activity; regulation of Ras protein signal transduction; regulation of smooth muscle contraction
Disease: Nephrotic Syndrome, Type 3
Research Articles on PLCE1
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Product Notes
The PLCE1 plce1 (Catalog #AAA9418236) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLCE1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLCE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:500-1:2000. Researchers should empirically determine the suitability of the PLCE1 plce1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLCE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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