Human Claudin 7 (CLDN7) ELISA Kit | CLDN7 elisa kit

Human Claudin 7 (CLDN7) ELISA Kit

Cat. Num. AAA7214904

• Gene Names: CLDN7; CLDN-7; CEPTRL2; CPETRL2; Hs.84359; claudin-1
• Reactivity: Human
• Synonyms: Claudin 7 (CLDN7); Human Claudin 7 (CLDN7) ELISA Kit; CLDN7 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLDN7 elisa kit

Intended Uses: This CLDN7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLDN7. This ELISA kit for research use only!

Principle of the Assay: CLDN7 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CLDN7 antibody and an CLDN7-HRP conjugate. The assay sample and buffer are incubated together with CLDN7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN7 concentration since CLDN7 from samples and CLDN7-HRP conjugate compete for the anti-CLDN7 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN7 from the sample, fewer sites are left to bind CLDN7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CLDN7 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 47938322
• NCBI GeneID: 1366
• Molecular Weight: 16,837 Da
• NCBI Official Full Name: Claudin 7
• NCBI Official Synonym Full Names: claudin 7
• NCBI Official Symbol: CLDN7
• NCBI Official Synonym Symbols: CLDN-7; CEPTRL2; CPETRL2; Hs.84359; claudin-1
• NCBI Protein Information: claudin-7; clostridium perfringens enterotoxin receptor-like 2
• UniProt Protein Name: Claudin-7
• Protein Family: Claudin
• UniProt Gene Name: CLDN7
• UniProt Synonym Gene Names: CEPTRL2; CPETRL2; CLDN-7
• UniProt Entry Name: CLD7_HUMAN

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc.. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]

Uniprot Description

Claudin-7: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: tight junction; basolateral plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: identical protein binding; protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion