Secreted Phospholipase A2-XII Recombinant Protein | PLA2G12 recombinant protein

Recombinant Human Secreted Phospholipase A2-XII

Cat. Num. AAA142471

• Gene Names: PLA2G12A; GXII; ROSSY; PLA2G12
• Applications: Western Blot
• Purity: Greater than 95% as determined by SDS PAGE.
• Synonyms: Secreted Phospholipase A2-XII; Recombinant Human Secreted Phospholipase A2-XII; PLA2G12 Human; Secreted Phospholipase A2-XII Human Recombinant; Group XIIA secretory phospholipase A2; EC 3.1.1.4; Phosphatidylcholine 2-acylhydrolase GXII; GXII sPLA2; PLA2G12; sPLA2-XII; PLA2G12A; PLA2G12 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95% as determined by SDS PAGE.
• Form/Format: Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 0.01M Tris buffer pH 8.6.; Filtered White lyophilized (freeze-dried) powder.
• Sequence: MRGSHHHHHH GMASHMQEQA QTTDWRATLK TIRNGVHKID TYLNAALDLL GGEDGLCQYK CSDGSKPFPR YGYKPSPPNG CGSPLFGVHLNIGIPSLTKC CNQHDRCYET CGKSKNDCDE EFQYCLSKIC RDVQKTLGLTQ HVQACETTVE LLFDSVIHLG CKPYLDSQRAACRCHYEEKT DL
• Sequence Length: 189

• Applicable Applications for PLA2G12 recombinant protein: Western Blot (WB)
• Solubility: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for PLA2G12 recombinant protein

Introduction: Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators.The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of low molecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso- PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.

Product Categories/Family for PLA2G12 recombinant protein

Enzymes; Secreted Phospholipase A2

NCBI and Uniprot Product Information

• NCBI GI #: 21361944
• NCBI GeneID: 81579
• NCBI Accession #: NP_110448.2
• NCBI GenBank Nucleotide #: NM_030821.4
• UniProt Accession #: Q9BZM1; Q9BZ89
• Molecular Weight: 21,067 Da
• NCBI Official Full Name: group XIIA secretory phospholipase A2
• NCBI Official Synonym Full Names: phospholipase A2, group XIIA
• NCBI Official Symbol: PLA2G12A
• NCBI Official Synonym Symbols: GXII; ROSSY; PLA2G12
• NCBI Protein Information: group XIIA secretory phospholipase A2; GXII sPLA2; group XII secreted phospholipase A2; group XIIA secreted phospholipase A2; phosphatidylcholine 2-acylhydrolase 12A; phospholipase A2, group XII; sPLA2-XII
• UniProt Protein Name: Group XIIA secretory phospholipase A2
• UniProt Gene Name: PLA2G12A
• UniProt Synonym Gene Names: PLA2G12; GXII sPLA2; sPLA2-XII
• UniProt Entry Name: PG12A_HUMAN

NCBI Description

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM, Mar 2008]

Uniprot Description

PLA2G12A: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine. Belongs to the phospholipase A2 family.

Protein type: Phospholipase; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Secreted, signal peptide; Endoplasmic reticulum; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Secreted; Lipid Metabolism - alpha-linolenic acid

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Golgi apparatus; endoplasmic reticulum; extracellular region

Molecular Function: calcium-dependent phospholipase A2 activity; calcium ion binding

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process