SDS-Page
(SDS-PAGE of 60kDa Hsp60 protein (SPR-104).)
Hsp60 Active Protein | HSPD1 active protein
Recombinant Human HSP60
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
Hsp60; Recombinant Human HSP60; 60kDa chaperonin; cb863; CPN60; GROEL; GroEL Homolog; HLD4; Hsp65; HSPD1; HuCHA60; SPG13; HSPD1 active protein
Host
Human
Reactivity
Human
Form/Format
Multi-step Chromatographically Purified
Concentration
0.47mg/mL (varies by lot)
Sequence Length
2247
Applicable Applications for HSPD1 active protein
ATPase Assay, Western Blot control (WB), Binding Assay, ELISA (EIA) reference standard
Certificate of Analysis
This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.6uM phosphate liberated/hr/ug protein in a 200ul reaction at 37 degree C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
Conjugate Tag
His tag
Expression System
E Coli
Storage Buffer
20mM Phosphate Buffer, 150mM NaCl, 10% glycerol
Preparation and Storage
-20 degree C
SDS-Page
(SDS-PAGE of 60kDa Hsp60 protein (SPR-104).)
SDS-Page
(SDS-PAGE of 60kDa Hsp60 protein (SPR-104).)
Related Product Information for HSPD1 active protein
Background Info: Molecular weight of approximately 60kDa
ATPase active
Scientific Background: In both prokaryotic and eukaryotic cells, the misfolding In both prokaryotic and eukaryotic cells, the misfolding
ATPase active
Scientific Background: In both prokaryotic and eukaryotic cells, the misfolding In both prokaryotic and eukaryotic cells, the misfolding
Product Categories/Family for HSPD1 active protein
References
1. Hartl, F.U. (1996) Nature 381: 571-579. 2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366. 3.Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852-1858. 4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279- 2283. 5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7.Gupta, S. and Knowlton, A.A. J. Cell Mol, Med. 9: 51-58. 8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128. 9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297.
1. Dieude, M. et al. (2011). Association of autoantibodies to heat-shock protein 60 with arterial vascular events in patients with antiphospholipid antibodies. Arthritis & Rheumatism. 63 (8), 2416-2424. doi: 10.1002/art.30411
2. Kaiser, F., Steptoe, A., Thompson, S. and Henderson, B. (2014). Monocyte cytokine synthesis in response to extracellular cell stress proteins suggests these proteins exhibit network behaviour. Cell Stress & Chaperones. doi: 10.1007/s12192-013-0440-0
3. Adamus, G. et. al. (2013). Detection of autoantibodies against heat shock proteins and collapsin response
mediator proteins in autoimmune retinopathy. BMC Ophthalmology 2013, 13:48 doi:10.1186/1471-2415-13-48
1. Dieude, M. et al. (2011). Association of autoantibodies to heat-shock protein 60 with arterial vascular events in patients with antiphospholipid antibodies. Arthritis & Rheumatism. 63 (8), 2416-2424. doi: 10.1002/art.30411
2. Kaiser, F., Steptoe, A., Thompson, S. and Henderson, B. (2014). Monocyte cytokine synthesis in response to extracellular cell stress proteins suggests these proteins exhibit network behaviour. Cell Stress & Chaperones. doi: 10.1007/s12192-013-0440-0
3. Adamus, G. et. al. (2013). Detection of autoantibodies against heat shock proteins and collapsin response
mediator proteins in autoimmune retinopathy. BMC Ophthalmology 2013, 13:48 doi:10.1186/1471-2415-13-48
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60kD
NCBI Official Full Name
Homo sapiens heat shock 60kDa protein 1 (chaperonin), mRNA
NCBI Official Synonym Full Names
heat shock 60kDa protein 1 (chaperonin)
NCBI Official Symbol
HSPD1
NCBI Official Synonym Symbols
HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
NCBI Protein Information
60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
UniProt Protein Name
60 kDa heat shock protein, mitochondrial
Protein Family
UniProt Gene Name
HSPD1
UniProt Synonym Gene Names
HSP60; CPN60; HSP-60; Hsp60
UniProt Entry Name
CH60_HUMAN
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Product Notes
The HSPD1 hspd1 (Catalog #AAA806957) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human HSP60 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp60 can be used in a range of immunoassay formats including, but not limited to, ATPase Assay, Western Blot control (WB), Binding Assay, ELISA (EIA) reference standard. Researchers should empirically determine the suitability of the HSPD1 hspd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp60, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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