Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Phospholipase A2 Polyclonal Antibody | anti-PLA2G12 antibody
Polyclonal Antibody to Phospholipase A2, Group XII (PLA2G12)
Gene Names
Pla2g12a; GXII; Rossy; GXII-1; Pla2g12; mGXII-1; GXII-1-PLA2; 2310004B05Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Phospholipase A2; Polyclonal Antibody; Polyclonal Antibody to Phospholipase A2; Group XII (PLA2G12); PLA2G12A; sPLA2-XII; GXII sPLA2; Group XIIA secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 12A; anti-PLA2G12 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against PLA2G12. It has been selected for its ability to recognize PLA2G12 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
145
Applicable Applications for anti-PLA2G12 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
PLA2G12 (Gln26~Leu192)
Conjugate
No Conjugate
Cross Reactivity
Mouse
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant PLA2G12 (Gln26~Leu192) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073121
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,199 Da
NCBI Official Full Name
group XIIA secretory phospholipase A2 isoform 1
NCBI Official Synonym Full Names
phospholipase A2, group XIIA
NCBI Official Symbol
Pla2g12a
NCBI Official Synonym Symbols
GXII; Rossy; GXII-1; Pla2g12; mGXII-1; GXII-1-PLA2; 2310004B05Rik
NCBI Protein Information
group XIIA secretory phospholipase A2
UniProt Protein Name
Group XIIA secretory phospholipase A2
Protein Family
UniProt Gene Name
Pla2g12a
UniProt Synonym Gene Names
Pla2g12; GXII sPLA2; sPLA2-XII
Uniprot Description
PLA2G12A: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine. Belongs to the phospholipase A2 family.
Protein type: EC 3.1.1.4; Endoplasmic reticulum; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - arachidonic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Phospholipase; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3|3 G3
Cellular Component: cytoplasm; endoplasmic reticulum; extracellular region; Golgi apparatus; intracellular
Molecular Function: calcium ion binding; hydrolase activity; metal ion binding; phospholipase A2 activity
Biological Process: arachidonic acid secretion; lipid catabolic process; lipid metabolic process; phospholipid metabolic process
Similar Products
Product Notes
The PLA2G12 pla2g12a (Catalog #AAA2034093) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phospholipase A2, Group XII (PLA2G12) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase A2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the PLA2G12 pla2g12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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