Typical Testing Data/Standard Curve (for reference only)
Chicken Gamma Glutamyltransferase 1 ELISA Kit | gGT1 elisa kit
Chicken Gamma Glutamyltransferase 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GGT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GGT1 antibody and an GGT1-HRP conjugate. The assay sample and buffer are incubated together with GGT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GGT1 concentration since GGT1 from samples and GGT1-HRP conjugate compete for the anti-GGT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by GGT1 from the sample, fewer sites are left to bind GGT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GGT1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Oct 2008]
Uniprot Description
GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - cyanoamino acid; Membrane protein, integral; EC 3.4.19.14; Transferase; Other Amino Acids Metabolism - selenoamino acid; EC 3.4.19.13; EC 2.3.2.2
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: anchored to external side of plasma membrane; extracellular space; integral to membrane; plasma membrane
Molecular Function: protein binding; gamma-glutamyltransferase activity
Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; glutathione catabolic process; regulation of inflammatory response; glutathione biosynthetic process; arachidonic acid metabolic process; zymogen activation; spermatogenesis
Disease: Glutathionuria
Research Articles on gGT1
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Product Notes
The Chicken gGT1 ggt1 (Catalog #AAA738632) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA738632 ELISA Kit recognizes Chicken gGT1. It is sometimes possible for the material contained within the vial of "Gamma Glutamyltransferase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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