Guinea Pig IL-1beta ELISA Kit | IL-1beta elisa kit
Guinea pig IL-1beta (Interleukin 1 Beta) ELISA Kit
Gene Names
IL1B2; IL1B; IL-1beta
Reactivity
Guinea Pig
Synonyms
IL-1beta; Guinea pig IL-1beta (Interleukin 1 Beta) ELISA Kit; IL-1beta elisa kit
Reactivity
Guinea Pig
Specificity
This kit recognizes Guinea pig IL-1beta in samples. No significant cross-reactivity or interference between Guinea pig IL-1beta and analogues was observed.
Sequence Length
267
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
9.375pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Guinea pig IL-1beta were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Guinea pig IL-1beta were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-1beta elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Guinea pig IL-1beta concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IL-1beta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig IL-1beta and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Guinea pig IL-1beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Guinea pig IL-1beta. You can calculate the concentration of Guinea pig IL-1beta in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IL-1beta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig IL-1beta and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Guinea pig IL-1beta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Guinea pig IL-1beta. You can calculate the concentration of Guinea pig IL-1beta in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin 1, beta
NCBI Official Symbol
IL1B2
NCBI Official Synonym Symbols
IL1B; IL-1beta
NCBI Protein Information
interleukin 1, beta; interleukin-1 beta
