Mouse Polycystin-1 ELISA Kit | Pkd1 elisa kit

Mouse Polycystin-1 ELISA Kit

Cat. Num. AAA2882330

• Gene Names: Pkd1; PC1; mFLJ00285
• Reactivity: Mouse
• Synonyms: Polycystin-1; Mouse Polycystin-1 ELISA Kit; Autosomal dominant polycystic kidney disease 1 protein homolog; Pkd1; Pkd1 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse Polycystin-1
• Sequence Length: 4293

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.78-50 ng/mL
• Sensitivity: < 0.39 ng/mL
• Intra-assay Precision: <=7.1%
• Inter-assay Precision: <=11.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Pkd1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 124487380
• NCBI GeneID: 18763
• NCBI Accession #: NP_038658.2
• NCBI GenBank Nucleotide #: NM_013630.2
• UniProt Accession #: O08852; E9QJR6
• Molecular Weight: 466,577 Da
• NCBI Official Full Name: polycystin-1
• NCBI Official Synonym Full Names: polycystic kidney disease 1 homolog
• NCBI Official Symbol: Pkd1
• NCBI Official Synonym Symbols: PC1; mFLJ00285
• NCBI Protein Information: polycystin-1
• UniProt Protein Name: Polycystin-1
• Protein Family: Polycystin
• UniProt Gene Name: Pkd1
• UniProt Entry Name: PKD1_MOUSE

Uniprot Description

PKD1: Involved in renal tubulogenesis. Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. Acts as a regulator of cilium length, together with PKD2. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. May be an ion- channel regulator. Involved in adhesive protein-protein and protein-carbohydrate interactions. Defects in PKD1 are the cause of polycystic kidney disease autosomal dominant type 1 (ADPKD1). ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. Its prevalence is estimated at about 1/1000. Belongs to the polycystin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral

Cellular Component: basolateral plasma membrane; cell projection; cell surface; cilium; cytoplasm; cytosol; Golgi apparatus; integral to membrane; lateral plasma membrane; membrane; motile primary cilium; nucleus; plasma membrane

Molecular Function: calcium channel activity; carbohydrate binding; cation channel activity; protein binding; protein domain specific binding; protein kinase binding

Biological Process: blood vessel development; branching morphogenesis of a tube; calcium ion transport; cartilage condensation; cell cycle arrest; cell-cell adhesion; cytoplasmic sequestering of transcription factor; detection of mechanical stimulus; elevation of cytosolic calcium ion concentration; embryonic placenta development; establishment of cell polarity; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; heart development; in utero embryonic development; JAK-STAT cascade; kidney development; liver development; nitrogen compound metabolic process; peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of transcription from RNA polymerase II promoter; protein export from nucleus; regulation of cell adhesion