Mouse Protein Kinase C Epsilon ELISA Kit | PKCe elisa kit

Mouse Protein Kinase C Epsilon ELISA Kit

Cat. Num. AAA764231

• Gene Names: PRKCE; PKCE; nPKC-epsilon
• Reactivity: Mouse
• Synonyms: Protein Kinase C Epsilon; Mouse Protein Kinase C Epsilon ELISA Kit; PKCepsilon (Protein Kinase C Epsilon )/PRKCE/PKCE/nPKC-epsilon/MGC125656/PKCEMGC125657/protein kinase C epsilon type/protein kinase C; epsilon; PKCe elisa kit

• Reactivity: Mouse
• Sequence Length: 737

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PKCe elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse PKCe antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse PKCe antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PKCe amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse PKCe can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4885563
• NCBI GeneID: 5581
• NCBI Accession #: NP_005391.1
• NCBI GenBank Nucleotide #: NM_005400.2
• Molecular Weight: 83,674 Da
• NCBI Official Full Name: protein kinase C epsilon type
• NCBI Official Synonym Full Names: protein kinase C epsilon
• NCBI Official Symbol: PRKCE
• NCBI Official Synonym Symbols: PKCE; nPKC-epsilon
• NCBI Protein Information: protein kinase C epsilon type
• UniProt Protein Name: Protein kinase C epsilon type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCE
• UniProt Synonym Gene Names: PKCE
• UniProt Entry Name: KPCE_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.13; AGC group; PKC family; Eta subfamily

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: actin monomer binding; ATP binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; metal ion binding; protein binding; protein kinase C activity; protein serine/threonine kinase activity; receptor activator activity; signal transducer activity

Biological Process: apoptosis; blood coagulation; cell adhesion; cell cycle; cell division; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; lipopolysaccharide-mediated signaling pathway; macrophage activation during immune response; negative regulation of protein ubiquitination; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; phospholipase C activation; platelet activation; positive regulation of actin filament polymerization; positive regulation of cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of insulin secretion; positive regulation of lipid catabolic process; positive regulation of MAPKKK cascade; positive regulation of synaptic transmission, GABAergic; protein amino acid phosphorylation; regulation of peptidyl-tyrosine phosphorylation; regulation of release of sequestered calcium ion into cytosol; release of sequestered calcium ion into cytosol; response to morphine; signal transduction