Chicken Protein Kinase C Epsilon ELISA Kit | PKCe elisa kit

Chicken Protein Kinase C Epsilon ELISA Kit

Cat. Num. AAA743856

• Gene Names: PRKCE; PKCE; nPKC-epsilon
• Reactivity: Chicken
• Synonyms: Protein Kinase C Epsilon; Chicken Protein Kinase C Epsilon ELISA Kit; PKCe elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKCe elisa kit

Intended Uses: This PKCe ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PKCe. This ELISA kit for research use only!

Principle of the Assay: PKCe ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PKCe antibody and an PKCe-HRP conjugate. The assay sample and buffer are incubated together with PKCe-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PKCe concentration since PKCe from samples and PKCe-HRP conjugate compete for the anti-PKCe antibody binding site. Since the number of sites is limited, as more sites are occupied by PKCe from the sample, fewer sites are left to bind PKCe-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PKCe concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PKCe elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4885563
• NCBI GeneID: 5581
• NCBI Accession #: NP_005391.1
• NCBI GenBank Nucleotide #: NM_005400.2
• UniProt Accession #: Q02156; Q32MQ3; Q53SL4; Q53SM5; Q9UE81; B0LPH7; Q53RT0
• Molecular Weight: 83,674 Da
• NCBI Official Full Name: protein kinase C epsilon type
• NCBI Official Synonym Full Names: protein kinase C, epsilon
• NCBI Official Symbol: PRKCE
• NCBI Official Synonym Symbols: PKCE; nPKC-epsilon
• NCBI Protein Information: protein kinase C epsilon type
• UniProt Protein Name: Protein kinase C epsilon type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCE
• UniProt Synonym Gene Names: PKCE
• UniProt Entry Name: KPCE_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.

Protein type: Protein kinase, AGC; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; PKC family; Eta subfamily

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: Golgi apparatus; cytoskeleton; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: actin monomer binding; protein serine/threonine kinase activity; calcium-independent protein kinase C activity; protein binding; signal transducer activity; enzyme binding; protein kinase C activity; metal ion binding; enzyme activator activity; ethanol binding; receptor activator activity; ATP binding

Biological Process: positive regulation of cytokinesis; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of synaptic transmission, GABAergic; positive regulation of insulin secretion; response to morphine; positive regulation of lipid catabolic process; cell cycle; signal transduction; protein amino acid phosphorylation; peptidyl-serine phosphorylation; positive regulation of MAPKKK cascade; positive regulation of actin filament polymerization; cell division; phospholipase C activation; release of sequestered calcium ion into cytosol; macrophage activation during immune response; lipopolysaccharide-mediated signaling pathway; innate immune response; cell adhesion; blood coagulation; regulation of peptidyl-tyrosine phosphorylation