bPhosphoinositide 3-kinase a Recombinant Protein | bP85a recombinant protein

Recombinant bovine Phosphoinositide 3-kinase a, regulatory subunit

Cat. Num. AAA142822

• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: bPhosphoinositide 3-kinase a; Recombinant bovine Phosphoinositide 3-kinase a; regulatory subunit; p85a Bovine; Phosphoinositide 3-kinase a; regulatory subunit Bovine Recombinant; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase p85 subunit alpha; PtdIns-3-kinase p85-alpha; PI3K; P85a; bP85a recombinant protein

• Host: Leishmania tarentolae
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: Supplied as a 1 mg/ml solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2 and 50% glycerol.; Sterile filtered liquid formualtion.
• Sequence Length: 724

• Preparation and Storage: P85a although stable at 4 degree C for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for bP85a recombinant protein

Description: Phosphoinositide 3-kinase subunit p85a Bovine Recombinant has a molecular weight of 83.5 kDa.

Introduction: P85a functions as the regulatory subunit of the class IA PI3-kinase isoforms a, b, and d. It contains two SH2 domains that bind to tyrosine-phosphorylated growth factor receptors or substrate adaptor proteins.It also contains a BH (breakpoint cluster region homology) domain that shows GAP activity towards the small GTPases Rab4, Rab5, Cdc42, Rac1 and to a lesser extend towards Rab6 and Rab11.It was shown that PI3Ka catalytic subunit mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3'-phosphorylated phosphoinositides.PI3Ka protein kinase activity has been implicated in IRS-1 serine phosphorylation in insulin-treated adipocytes and in STAT3 and IRS-1 phosphorylation upon activation of the type 1 IFN receptor by IFN-a.

Product Categories/Family for bP85a recombinant protein

Protein Kinases; Enzymes; Phosphoinositide 3-kinase

NCBI and Uniprot Product Information

• NCBI GI #: 27807039
• NCBI GeneID: 282307
• NCBI Accession #: NP_777000.1
• NCBI GenBank Nucleotide #: NM_174575.1
• UniProt Accession #: P23727
• Molecular Weight: 83,497 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit alpha
• NCBI Official Symbol: PIK3R1
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase p85 subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha); phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); ptdIns-3-kinase p85-alpha; ptdIns-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit p85-alpha
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• UniProt Gene Name: PIK3R1
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
• UniProt Entry Name: P85A_BOVIN

Uniprot Description

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement ().