Phosphoinositide 3 Kinase, p110 alpha, p85 alpha Active Protein | PIK3R1 active protein

Phosphoinositide 3 Kinase, Recombinant, Human, p110 alpha, p85 alpha (PI3K)

Cat. Num. AAA636424

• Gene Names: PIK3R1; p85; AGM7; GRB1; p85-ALPHA
• Reactivity: Human
• Purity: 80% by SDS-PAGE
• Synonyms: Phosphoinositide 3 Kinase; p110 alpha; p85 alpha; Recombinant; Human; p85 alpha (PI3K); PIK3R1 active protein

• Reactivity: Human
• Purity/Purification: 80% by SDS-PAGE
• Form/Format: Supplied as a liquid in 25mM Tris, pH 8.0, 69mM sodium chloride, 1.35mM KCl, 0.025% Tween-20, 3mM DTT, 50% glycerol

• Application Notes: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: ~600pmol/min/ug
• Assay Buffer: 40mM Tris-HCl, pH 7.5, 20mM MgCl2, 100uM ATP, 200uM PI/PS, 20-100ng P4073-26C for 30 min at 30ºC.
• Preparation and Storage: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.

Testing Data

(specific activity)

SDS-Page

(10% SDS-PAGE Coomassie Staining. Lane 1: 4ug P4073-26C. Lane 2: Protein Marker. Molecular Weight: p110a: 125kD. p85a: 84kD.)

Related Product Information for PIK3R1 active protein

Phosphoinositide 3 Kinase, Recombinant, Bovine, p85 alpha (PI3K) functions as the regulatory subunit of the class IA PI3-kinase isoforms a, b, and d. It contains two SH2 domains that bind to tyrosine-phosphorylated growth factor receptors or substrate adaptor proteins. It also contains a BH (breakpoint cluster region homology) domain that shows GAP activity towards the small GTPases Rab4, Rab5, Cdc42, Rac1 and to a lesser extend towards Rab6 and Rab11. It was shown that PI3Ka catalytic subunit mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3'-phosphorylated phosphoinositides. PI3Ka protein kinase activity has been implicated in IRS-1 serine phosphorylation in insulin-treated adipocytes and in STAT3 and IRS-1 phosphorylation upon activation of the type 1 IFN receptor by IFN-a.

Complex of N-terminal GST-tagged recombinant full-length human p110 alpha (GenBank Accession No. U79143), and N-terminal His tagged, recombinant full length, human p85alpha (XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.

Product Categories/Family for PIK3R1 active protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 32455250
• NCBI GeneID: 5295
• NCBI Accession #: NP_852665.1
• NCBI GenBank Nucleotide #: NM_181524.1
• UniProt Accession #: P27986; Q15747; Q4VBZ7; Q53EM6; Q8IXA2; Q8N1C5; B3KT19; D3DWA0; E7EX19
• Molecular Weight: 129, 88kD
• NCBI Official Full Name: phosphatidylinositol 3-kinase regulatory subunit alpha isoform 3
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
• NCBI Official Symbol: PIK3R1
• NCBI Official Synonym Symbols: p85; AGM7; GRB1; p85-ALPHA
• NCBI Protein Information: phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
• UniProt Protein Name: Phosphatidylinositol 3-kinase regulatory subunit alpha
• Protein Family: Phosphatidylinositol 3-kinase regulatory
• UniProt Gene Name: PIK3R1
• UniProt Synonym Gene Names: GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha
• UniProt Entry Name: P85A_HUMAN

NCBI Description

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Uniprot Description

PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Enzyme, regulatory subunit

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; nucleus; cytosol; phosphoinositide 3-kinase complex

Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; insulin receptor substrate binding; neurotrophin TRKA receptor binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; transmembrane receptor protein tyrosine kinase adaptor protein activity; transcription factor binding; protein phosphatase binding; insulin receptor binding

Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; viral reproduction; nerve growth factor receptor signaling pathway; protein amino acid phosphorylation; positive regulation of RNA splicing; regulation of phosphoinositide 3-kinase activity; NFAT protein import into nucleus; T cell receptor signaling pathway; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein stabilization; positive regulation of tumor necrosis factor production; negative regulation of cell-matrix adhesion; cellular response to insulin stimulus; induction of apoptosis via death domain receptors; insulin-like growth factor receptor signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; B cell differentiation; T cell costimulation; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; innate immune response; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; positive regulation of cell migration; negative regulation of apoptosis

Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36