Mouse Krueppel-like factor 10 ELISA Kit | Klf10 elisa kit

Mouse Krueppel-like factor 10 ELISA Kit

Cat. Num. AAA288056

• Gene Names: Klf10; Tieg; mGIF; Egral; Tieg1; EGR[a]; Gdnfif; TIEG-1; AI115143
• Reactivity: Mouse
• Synonyms: Krueppel-like factor 10; Mouse Krueppel-like factor 10 ELISA Kit; Klf10 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Krueppel-like factor 10. No significant cross-reactivity or interference was observed.
• Sequence Length: 469

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/ml
• Sensitivity: 39.2 pg/mL
• Intra-Assay CV: <=7.4%
• Inter-Assay CV: <=9.4%
• Recovery: 90%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Klf10 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Krueppel-like factor 10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Krueppel-like factor 10 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Krueppel-like factor 10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Krueppel-like factor 10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7305571
• NCBI GeneID: 21847
• NCBI Accession #: NP_038720.1
• NCBI GenBank Nucleotide #: NM_013692.3
• UniProt Accession #: O89091
• Molecular Weight: 51,756 Da
• NCBI Official Full Name: Krueppel-like factor 10 isoform 1
• NCBI Official Synonym Full Names: Kruppel-like factor 10
• NCBI Official Symbol: Klf10
• NCBI Official Synonym Symbols: Tieg; mGIF; Egral; Tieg1; EGR[a]; Gdnfif; TIEG-1; AI115143
• NCBI Protein Information: Krueppel-like factor 10
• UniProt Protein Name: Krueppel-like factor 10
• Protein Family: Krueppel-like factor
• UniProt Gene Name: Klf10
• UniProt Synonym Gene Names: Gdnfif; Tieg; Tieg1; mGIF; TGFB-inducible early growth response protein 1; TIEG-1
• UniProt Entry Name: KLF10_MOUSE

Uniprot Description

KLF10: Transcriptional repressor involved in the regulation of cell growth. Inhibits cell growth. Binds to the consensus sequence 5'-GGTGTG-3'. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; nucleic acid binding; protein binding

Biological Process: bone mineralization; cellular response to starvation; negative regulation of transcription, DNA-dependent; positive regulation of osteoclast differentiation; regulation of circadian rhythm; regulation of transcription, DNA-dependent; rhythmic process; somatic stem cell maintenance; transcription, DNA-dependent