Platelet Factor 4 (PF4) Recombinant Protein | PF4 recombinant protein

Recombinant Platelet Factor 4 (PF4)

Cat. Num. AAA2018453

• Gene Names: Pf4; PF-4; Pf4a; Cxcl4; RATPF4A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Platelet Factor 4 (PF4); Recombinant Platelet Factor 4 (PF4); PF4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-LLLG LLLLPAVVAV TRASPEESDG DLSCVCVKTS SSRIHLKRIT SLEVIKAGPH CAVPQLIATL KNGSKICLDRQVPLYKKIIK KLLE
• Sequence Length: 105

• Applicable Applications for PF4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu17~Glu104 (Accession # P06765) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 56090531
• NCBI GeneID: 360918
• NCBI Accession #: NP_001007730.1
• NCBI GenBank Nucleotide #: NM_001007729.1
• UniProt Accession #: P06765
• Molecular Weight: 41.9kDa
• NCBI Official Full Name: platelet factor 4
• NCBI Official Synonym Full Names: platelet factor 4
• NCBI Official Symbol: Pf4
• NCBI Official Synonym Symbols: PF-4; Pf4a; Cxcl4; RATPF4A
• NCBI Protein Information: platelet factor 4; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4
• UniProt Protein Name: Platelet factor 4
• Protein Family: Platelet factor
• UniProt Gene Name: Pf4
• UniProt Synonym Gene Names: Cxcl4; Scyb4; PF-4
• UniProt Entry Name: PLF4_RAT

NCBI Description

member of low-molecular weight chemokines superfamily; may mediate inflammation and immune response [RGD, Feb 2006]

Uniprot Description

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis; Apoptosis

Cellular Component: extracellular space; protein complex; vesicle

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding

Biological Process: negative regulation of cytolysis; platelet activation; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; negative regulation of MHC class II biosynthetic process; protein complex assembly; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of macrophage differentiation; inflammatory response