Human Nociceptin receptor ELISA Kit | OPRL1 elisa kit

Human Nociceptin receptor ELISA Kit

Cat. Num. AAA763161

• Gene Names: OPRL1; NOP; OOR; ORL1; KOR-3; NOCIR
• Reactivity: Human
• Synonyms: Nociceptin receptor; Human Nociceptin receptor ELISA Kit; OPRL1/Nociceptin receptor/Kappa-type 3 opioid receptor (KOR-3)/Orphanin FQ receptor; OPRL1 elisa kit

• Reactivity: Human
• Sequence Length: 370

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for OPRL1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human OPRL1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human OPRL1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human OPRL1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human OPRL1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 317108157
• NCBI GeneID: 4987
• NCBI Accession #: NP_001186948.1
• NCBI GenBank Nucleotide #: NM_001200019.1
• Molecular Weight: 40,048 Da
• NCBI Official Full Name: nociceptin receptor isoform 1
• NCBI Official Synonym Full Names: opiate receptor-like 1
• NCBI Official Symbol: OPRL1
• NCBI Official Synonym Symbols: NOP; OOR; ORL1; KOR-3; NOCIR
• NCBI Protein Information: nociceptin receptor
• UniProt Protein Name: Nociceptin receptor
• Protein Family: Nociceptin receptor
• UniProt Gene Name: OPRL1
• UniProt Synonym Gene Names: OOR; ORL1; KOR-3
• UniProt Entry Name: OPRX_HUMAN

NCBI Description

The protein encoded by this gene is a member of the 7 transmembrane-spanning G protein-coupled receptor family, and functions as a receptor for the endogenous, opioid-related neuropeptide, nociceptin/orphanin FQ. This receptor-ligand system modulates a variety of biological functions and neurobehavior, including stress responses and anxiety behavior, learning and memory, locomotor activity, and inflammatory and immune responses. A promoter region between this gene and the 5'-adjacent RGS19 (regulator of G-protein signaling 19) gene on the opposite strand functions bi-directionally as a core-promoter for both genes, suggesting co-operative transcriptional regulation of these two functionally related genes. Alternatively spliced transcript variants have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jan 2016]

Uniprot Description

KOR-3: Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: cytoplasmic membrane-bound vesicle; cytosol; integral to plasma membrane; neuron projection; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide binding; nociceptin/orphanin-FQ receptor activity; protein binding; protein C-terminus binding

Biological Process: eating behavior; elevation of cytosolic calcium ion concentration; G-protein signaling, adenylate cyclase inhibiting pathway; negative regulation of blood pressure; negative regulation of cAMP biosynthetic process; neuropeptide signaling pathway; positive regulation of protein amino acid phosphorylation; response to estradiol stimulus; sensory perception; sensory perception of pain; synaptic transmission