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Typical Testing Data

Human Protein disulfide-isomerase A4 ELISA Kit | PDIA4 elisa kit

Human Protein disulfide-isomerase A4 ELISA Kit

Gene Names
PDIA4; ERP70; ERP72; ERp-72
Reactivity
Human
Synonyms
Protein disulfide-isomerase A4; Human Protein disulfide-isomerase A4 ELISA Kit; PDIA4/Protein disulfide-isomerase A4/Endoplasmic reticulum resident protein 70/ER protein 70/ERp70/Endoplasmic reticulum resident protein 72/ER protein 72/ERp-72/ERp72; PDIA4 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
645
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
1.56-100ng/ml
Sensitivity
0.938ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for PDIA4 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PDIA4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PDIA4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PDIA4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PDIA4 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,932 Da
NCBI Official Full Name
protein disulfide-isomerase A4
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 4
NCBI Official Symbol
PDIA4
NCBI Official Synonym Symbols
ERP70; ERP72; ERp-72
NCBI Protein Information
protein disulfide-isomerase A4
UniProt Protein Name
Protein disulfide-isomerase A4
UniProt Gene Name
PDIA4
UniProt Synonym Gene Names
ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72
UniProt Entry Name
PDIA4_HUMAN

Uniprot Description

PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.

Protein type: Endoplasmic reticulum; Isomerase; EC 5.3.4.1; Chaperone

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: cell surface; endoplasmic reticulum lumen; melanosome

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: cell redox homeostasis; metabolic process; protein folding; protein secretion

Research Articles on PDIA4

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Product Notes

The Human PDIA4 pdia4 (Catalog #AAA760831) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA760831 ELISA Kit recognizes Human PDIA4. It is sometimes possible for the material contained within the vial of "Protein disulfide-isomerase A4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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