Human disulfide-isomerase A4 ELISA Kit | PDIA4 elisa kit

Human Protein disulfide-isomerase A4 ELISA Kit

Cat. Num. AAA9426481

• Gene Names: PDIA4; ERP70; ERP72; ERp-72
• Reactivity: Human
• Synonyms: disulfide-isomerase A4; Human Protein disulfide-isomerase A4 ELISA Kit; Protein disulfide-isomerase A4; Endoplasmic reticulum resident protein 70; ER protein 70; ERp70; Endoplasmic reticulum resident protein 72; ER protein 72; ERp-72; ERp72; PDIA4; ERP70; ERP72; PDIA4 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Protein bassoon. No significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenates And Cell Culture Supernates And Other Biological Fluids
• Detection Range: 78.0 pg/mL-5000 pg/mL
• Sensitivity: 36 pg/ml
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDIA4 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Protein bassoon concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein bassoon. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein bassoon and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein bassoon, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Protein bassoon in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758304
• NCBI GeneID: 9601
• NCBI Accession #: NP_004902.1
• NCBI GenBank Nucleotide #: NM_004911.4
• UniProt Accession #: P13667; Q549T6; A8K4K6
• Molecular Weight: 72,932 Da
• NCBI Official Full Name: protein disulfide-isomerase A4
• NCBI Official Synonym Full Names: protein disulfide isomerase family A member 4
• NCBI Official Symbol: PDIA4
• NCBI Official Synonym Symbols: ERP70; ERP72; ERp-72
• NCBI Protein Information: protein disulfide-isomerase A4
• UniProt Protein Name: Protein disulfide-isomerase A4
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA4
• UniProt Synonym Gene Names: ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72

NCBI Description

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016]

Uniprot Description

PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.

Protein type: Chaperone; EC 5.3.4.1; Endoplasmic reticulum; Isomerase

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: cell surface; endoplasmic reticulum; extracellular space

Molecular Function: peptide disulfide oxidoreductase activity; protein binding; protein disulfide isomerase activity; RNA binding

Biological Process: protein folding; protein secretion