PDGFRB / PDGFR-1 recombinant protein

Recombinant Rat PDGFRB / PDGFR-1 Protein (His tag)

Cat. Num. AAA2547327

• Gene Names: Pdgfrb; PDGFR-1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: PDGFRB / PDGFR-1; Recombinant Rat PDGFRB / PDGFR-1 Protein (His tag); PDGFRB; Pdgfr; Pdgfr1; PDGFRB / PDGFR-1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1097

• Application Notes: The recombinant rat PDGFRB comprises 510 amino acids and predicts a molecular mass of 57.6 kDa. The apparent molecular mass of the recombinant protein is approximately 92 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Leu 32
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PDGFRB / PDGFR-1 recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-beta was correlated with depth of cancer invasion at statistically significant level.

Description: A DNA sequence encoding the rat PDGFRB (Q05030) (Met1-Lys530) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 29789275
• NCBI GeneID: 24629
• NCBI Accession #: NP_113713.1
• NCBI GenBank Nucleotide #: NM_031525.1
• UniProt Accession #: Q05030; Q8R406; Q925F7
• Molecular Weight: 122,828 Da
• NCBI Official Full Name: platelet-derived growth factor receptor beta
• NCBI Official Synonym Full Names: platelet derived growth factor receptor beta
• NCBI Official Symbol: Pdgfrb
• NCBI Official Synonym Symbols: PDGFR-1
• NCBI Protein Information: platelet-derived growth factor receptor beta
• UniProt Protein Name: Platelet-derived growth factor receptor beta
• UniProt Gene Name: Pdgfrb
• UniProt Synonym Gene Names: Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1

NCBI Description

binds platelet derived growth factor BB homodimer [RGD, Feb 2006]

Uniprot Description

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group