CD140b / PDGFRB recombinant protein

Recombinant Human CD140b / PDGFRB Protein (His tag)

Cat. Num. AAA2546211

• Gene Names: PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CD140b / PDGFRB; Recombinant Human CD140b / PDGFRB Protein (His tag); PDGFRB; CD140B; JTK12; PDGFR; PDGFR1; CD140b / PDGFRB recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1106

• Application Notes: The secreted recombinant human PDGFRbeta comprises 510 amino acids and predicts a molecular mass of 57.6 kDa. It migrates as an approximately 95-100 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Leu 33
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD140b / PDGFRB recombinant protein

Background: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-beta was correlated with depth of cancer invasion at statistically significant level.

Description: A DNA sequence encoding the human PDGFRbeta (NP_002600.1) extracellular domain (Met 1-Lys 531) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 4505683
• NCBI GeneID: 5159
• NCBI Accession #: NP_002600.1
• NCBI GenBank Nucleotide #: NM_002609.3
• UniProt Accession #: P09619; Q8N5L4; B5A957
• Molecular Weight: 37,412 Da
• NCBI Official Full Name: platelet-derived growth factor receptor beta
• NCBI Official Synonym Full Names: platelet derived growth factor receptor beta
• NCBI Official Symbol: PDGFRB
• NCBI Official Synonym Symbols: IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1
• NCBI Protein Information: platelet-derived growth factor receptor beta
• UniProt Protein Name: Platelet-derived growth factor receptor beta
• UniProt Gene Name: PDGFRB
• UniProt Synonym Gene Names: PDGFR; PDGFR1; PDGF-R-beta; PDGFR-beta; PDGFR-1

NCBI Description

This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: apical plasma membrane; cytoplasm; focal adhesion; intrinsic to plasma membrane; membrane; nucleus; plasma membrane

Molecular Function: enzyme binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; platelet activating factor receptor activity; platelet-derived growth factor beta-receptor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor activity; platelet-derived growth factor receptor binding; protein binding; protein kinase binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor binding

Biological Process: cardiac myofibril assembly; cell migration; MAPKKK cascade; peptidyl-tyrosine phosphorylation; phosphatidylinositol metabolic process; phosphoinositide-mediated signaling; platelet-derived growth factor receptor signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of phosphoprotein phosphatase activity; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; protein amino acid autophosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of phosphoinositide 3-kinase cascade; signal transduction

Disease: Basal Ganglia Calcification, Idiopathic, 1; Basal Ganglia Calcification, Idiopathic, 4; Kosaki Overgrowth Syndrome; Myeloproliferative Disorder, Chronic, With Eosinophilia; Myofibromatosis, Infantile, 1; Premature Aging Syndrome, Penttinen Type