PDGFA recombinant protein

PDGFA Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122746

• Purity: >95% as determined by SDS-PAGE
• Synonyms: PDGFA; PDGFA Protein; Mouse; Recombinant (His Tag); Mouse PDGFA Protein (His Tag); platelet-derived growth factor alpha polypeptide; PDGFA recombinant protein

• Host: Yeast
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 30% CAN, 0.1% TFA. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser87-Arg196

• Species: Mouse
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse PDGFA (NP_032834.1)(Ser87-Arg196) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PDGFA recombinant protein

Background: Platelet-derived growth factor alpha (PDGFA) is frequently upregulated in various cancers and thought to function as a key player in the development and progression of tumor growth by regulating aspects of cell proliferation, angiogenesis and metastasis. The human platelet-derived growth factor A chain gene (PDGFA) on chromosome 7p22 encodes an important mitogen. Within PDGFA lies a complex minisatellite structure that results in partial duplications of exon 4 and the IVS4 splice donor site.

NCBI and Uniprot Product Information

• NCBI GI #: 6715566
• NCBI GeneID: 18590
• NCBI Accession #: NP_032834.1
• NCBI GenBank Nucleotide #: NM_008808.3
• UniProt Accession #: P20033
• Molecular Weight: 22,267 Da
• NCBI Official Full Name: platelet-derived growth factor subunit A
• NCBI Official Synonym Full Names: platelet derived growth factor, alpha
• NCBI Official Symbol: Pdgfa
• NCBI Protein Information: platelet-derived growth factor subunit A; PDGF subunit A; PDGF-1; platelet-derived growth factor A chain; platelet-derived growth factor alpha polypeptide
• UniProt Protein Name: Platelet-derived growth factor subunit A
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: Pdgfa
• UniProt Synonym Gene Names: PDGF subunit A
• UniProt Entry Name: PDGFA_MOUSE

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cytokine; Cell cycle regulation

Cellular Component: extracellular space; microvillus; cell surface; membrane; extracellular region

Molecular Function: collagen binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; multicellular organismal development; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; regulation of smooth muscle cell migration; hair follicle development; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; organ morphogenesis; gut development; positive regulation of cell division; blood coagulation; actin cytoskeleton organization and biogenesis; alveolus development; positive regulation of DNA replication; positive regulation of cell migration