PDE2A recombinant protein

PDE2A Protein, Human, Recombinant (aa 215-900, His Tag)

Cat. Num. AAA8121249

• Gene Names: PDE2A; PDE2A1; PED2A4; cGSPDE; CGS-PDE
• Purity: >90% as determined by SDS-PAGE
• Synonyms: PDE2A; PDE2A Protein; Human; Recombinant (aa 215-900; His Tag); Human PDE2A/CGS-PDE Protein (aa 215-900; CGS-PDE Protein; cGSPDE Protein; PDE2A1 Protein; PED2A4 Protein; phosphodiesterase 2A; cGMP-stimulated; PDE2A recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu215-His900

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the amino acids (Glu 215-His 900) of human PDE2A (O00408-1) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PDE2A recombinant protein

Background: cGMP-dependent 3',5'-cyclic phosphodiesterase, also known as cyclic GMP-stimulated phosphodiesterase and PDE2A, is a peripheral membrane protein that belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. Phosphodiesterases (PDEs) comprise a family of enzymes that regulate the levels of cyclic nucleotides, key second messengers that mediate a diverse array of functions. Phosphodiesterases (PDEs) modulate signaling by cyclic nucleotides in diverse processes such as cardiac contractility, platelet aggregation, lipolysis, glycogenolysis, and smooth muscle contraction. PDE2A is an evolutionarily conserved cGMP-stimulated cAMP and cGMP PDE. PDE2A contains two GAF domains. PDE2A is expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. PDE2A is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. PDE2A is involved in the regulation of blood pressure and fluid homeostasis by the atrial natriuretic peptide (ANP), making PDE2-type enzymes important targets for drug discovery.

References

Iffland,A. et al., 2005, Biochemistry. 44 (23):8312-25 de Oliveira,S.K. et al., 2007,J Biol Chem. 282 (18):13656-63. Stephenson,D.T. et al., 2009, J Histochem Cytochem. 57 (10):933-49. Russwurm,C. et al., 2009, J Biol Chem. 284 (38):25782-90.

NCBI and Uniprot Product Information

• NCBI GI #: 219802706
• NCBI GeneID: 5138
• NCBI Accession #: NP_001137311.1
• NCBI GenBank Nucleotide #: NM_001143839.3
• UniProt Accession #: O00408; Q5J791; Q5J792; Q5J793; Q6ZMR1; B2R646; B3KRV5; E9PGI1; F6W5Z0
• Molecular Weight: 87,235 Da
• NCBI Official Full Name: cGMP-dependent 3',5'-cyclic phosphodiesterase isoform PDE2A2
• NCBI Official Synonym Full Names: phosphodiesterase 2A, cGMP-stimulated
• NCBI Official Symbol: PDE2A
• NCBI Official Synonym Symbols: PDE2A1; PED2A4; cGSPDE; CGS-PDE
• NCBI Protein Information: cGMP-dependent 3',5'-cyclic phosphodiesterase; cGMP-stimulated phosphodiesterase 1; cGMP-stimulated phosphodiesterase 4; cyclic GMP-stimulated phosphodiesterase
• UniProt Protein Name: cGMP-dependent 3',5'-cyclic phosphodiesterase
• Protein Family: cGMP-dependent 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE2A
• UniProt Synonym Gene Names: CGS-PDE; cGSPDE
• UniProt Entry Name: PDE2A_HUMAN

Uniprot Description

PDE2A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Phosphodiesterase; EC 3.1.4.17; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: presynaptic membrane; Golgi apparatus; mitochondrial matrix; axon; endoplasmic reticulum; perinuclear region of cytoplasm; dendrite; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; TPR domain binding; calcium channel activity; metal ion binding; cGMP-stimulated cyclic-nucleotide phosphodiesterase activity; cGMP binding; cyclic-nucleotide phosphodiesterase activity; drug binding; cAMP binding

Biological Process: monocyte differentiation; metabolic process; cGMP-mediated signaling; cAMP catabolic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of vascular permeability; cAMP-mediated signaling; negative regulation of cAMP biosynthetic process; negative regulation of protein import into nucleus, translocation; negative regulation of vascular permeability; regulation of cGMP metabolic process; cGMP catabolic process; blood coagulation; protein targeting to mitochondrion; positive regulation of inflammatory response