Typical Testing Data/Standard Curve (for reference only)
Human Procalcitonin ELISA Kit | PCT elisa kit
QuicKey Human PCT (Procalcitonin) ELISA Kit
Reactivity
Human
Synonyms
Procalcitonin; QuicKey Human PCT (Procalcitonin) ELISA Kit; PCT elisa kit
Reactivity
Human
Specificity
This kit recognizes Human PCT in samples. No significant cross-reactivity or interference between Human PCT and analogues was observed.
Samples
Serum, plasma, urine. please consult technical support for the applicability if other biological fluids need to be tested
Assay Type
Quantitative Sandwich
Detection Range
0.31-20ng/mL
Sensitivity
0.08ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PCT were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PCT were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PCT elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PCT concentrations in serum, plasma, urine. Please consult technical support for the applicability if other biological fluids need to be tested.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCT. Samples (or Standards) and biotinylated detection antibody specific for Human PCT are added to the micro ELISA plate wells. Human PCT would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human PCT. You can calculate the concentration of Human PCT in the samples by comparing the OD of the samples to the standard curve.
Background/Introduction: Procalcitonin (PCT), the precursor of the hormone calcitonin, comprises 116 amino acids and is present in minute quantities under healthy conditions[1]. PCT and other calcitonin precursors in serum are present at less than 50 pg/mL in healthy individuals, but are highly elevated in serum where conditions leading to systemic inflammatory response syndrome or sepsis prevail. The level of PCT has been observed to increase significantly with bacterial infection and correlate to the severity of the infection. Therefore, serum PCT has been considered a powerful biomarker for the identification of bacterial infection[2]. PCT has been proven to have high accuracy as a marker of infection in adult patients, particularly in sepsis. In children it has been investigated in several conditions, including neonatal sepsis, severe infection in neutropenic children, and other specific bacterial infections (e.g. urinary tract infections, pneumonia, bone and joint tissue infections).
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PCT. Samples (or Standards) and biotinylated detection antibody specific for Human PCT are added to the micro ELISA plate wells. Human PCT would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 +/- 2 nm. The OD value is proportional to the concentration of Human PCT. You can calculate the concentration of Human PCT in the samples by comparing the OD of the samples to the standard curve.
Background/Introduction: Procalcitonin (PCT), the precursor of the hormone calcitonin, comprises 116 amino acids and is present in minute quantities under healthy conditions[1]. PCT and other calcitonin precursors in serum are present at less than 50 pg/mL in healthy individuals, but are highly elevated in serum where conditions leading to systemic inflammatory response syndrome or sepsis prevail. The level of PCT has been observed to increase significantly with bacterial infection and correlate to the severity of the infection. Therefore, serum PCT has been considered a powerful biomarker for the identification of bacterial infection[2]. PCT has been proven to have high accuracy as a marker of infection in adult patients, particularly in sepsis. In children it has been investigated in several conditions, including neonatal sepsis, severe infection in neutropenic children, and other specific bacterial infections (e.g. urinary tract infections, pneumonia, bone and joint tissue infections).
NCBI and Uniprot Product Information
NCBI GI #
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Product Notes
The Human PCT (Catalog #AAA2563941) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2563941 ELISA Kit recognizes Human PCT. It is sometimes possible for the material contained within the vial of "Procalcitonin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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