Typical Testing Data/Standard Curve (for reference only)
Bovine Procollagen C peptidase ELISA Kit | PCP elisa kit
Bovine Procollagen C peptidase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PCP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PCP antibody and an PCP-HRP conjugate. The assay sample and buffer are incubated together with PCP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCP concentration since PCP from samples and PCP-HRP conjugate compete for the anti-PCP antibody binding site. Since the number of sites is limited, as more sites are occupied by PCP from the sample, fewer sites are left to bind PCP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]
Uniprot Description
BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Protease; EC 3.4.24.19
Chromosomal Location of Human Ortholog: 8p21.3
Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; extracellular region
Molecular Function: peptidase activity; growth factor activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; cytokine activity; calcium ion binding
Biological Process: extracellular matrix disassembly; ossification; extracellular matrix organization and biogenesis; multicellular organismal development; lipoprotein metabolic process; proteolysis; cell differentiation; skeletal development; cartilage condensation
Disease: Osteogenesis Imperfecta, Type Xiii
Research Articles on PCP
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Product Notes
The Bovine PCP bmp1 (Catalog #AAA748304) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA748304 ELISA Kit recognizes Bovine PCP. It is sometimes possible for the material contained within the vial of "Procollagen C peptidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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