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Quality Control (Western blot analysis of immunized recombinant protein, using anti-STK3 monoclonal antibody. )

Mouse anti-Human STK3 Monoclonal Antibody | anti-STK3 antibody

Mouse monoclonal antibody Anti-Human STK3

Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Western Blot
Synonyms
STK3; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human STK3; Homo sapiens serine/threonine kinase 3 (STE20 homolog; yeast) (STK3); mRNA.; KRS1; MST2; FLJ90748; anti-STK3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3067C3a
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Applicable Applications for anti-STK3 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-STK3 monoclonal antibody. )

Quality Control (Western blot analysis of immunized recombinant protein, using anti-STK3 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Western Blot (WB)

(Detection of STK3 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3067C3a-1]Predicted molecular weight: 56 kDa)

Western Blot (WB) (Detection of STK3 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3067C3a-1]Predicted molecular weight: 56 kDa)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-STK3 mAb. [Lot No.3067C3a-1])

Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-STK3 mAb. [Lot No.3067C3a-1])
Related Product Information for anti-STK3 antibody
Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]). [supplied by OMIM][NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

NCBI Accession #
NCBI GenBank Nucleotide #

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Product Notes

The STK3 (Catalog #AAA120451) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human STK3 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STK3 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB). Researchers should empirically determine the suitability of the STK3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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