Sheep Bone Morphogenetic Protein 1 ELISA Kit | BMP1 elisa kit

Sheep Bone Morphogenetic Protein 1 ELISA Kit

Cat. Num. AAA736613

• Gene Names: BMP1; PCP; TLD; OI13; PCP2; PCOLC
• Reactivity: Sheep
• Synonyms: Bone Morphogenetic Protein 1; Sheep Bone Morphogenetic Protein 1 ELISA Kit; BMP1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This BMP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep BMP1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP1 elisa kit

Principle of the Assay: BMP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMP1 antibody and an BMP1-HRP conjugate. The assay sample and buffer are incubated together with BMP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMP1 concentration since BMP1 from samples and BMP1-HRP conjugate compete for the anti-BMP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BMP1 from the sample, fewer sites are left to bind BMP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMP1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BMP1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5453579
• NCBI GeneID: 649
• NCBI Accession #: NP_006120.1
• NCBI GenBank Nucleotide #: NM_006129.4
• Molecular Weight: 92,655 Da
• NCBI Official Full Name: bone morphogenetic protein 1 isoform 3
• NCBI Official Synonym Full Names: bone morphogenetic protein 1
• NCBI Official Symbol: BMP1
• NCBI Official Synonym Symbols: PCP; TLD; OI13; PCP2; PCOLC
• NCBI Protein Information: bone morphogenetic protein 1; mammalian tolloid protein; procollagen C-proteinase 3; procollagen C-endopeptidase
• UniProt Protein Name: Bone morphogenetic protein 1
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP1
• UniProt Synonym Gene Names: PCOLC; BMP-1; mTld; PCP
• UniProt Entry Name: BMP1_HUMAN

NCBI Description

This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]

Uniprot Description

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; EC 3.4.24.19; Protease

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: peptidase activity; growth factor activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; cytokine activity; calcium ion binding

Biological Process: extracellular matrix disassembly; ossification; extracellular matrix organization and biogenesis; multicellular organismal development; lipoprotein metabolic process; proteolysis; cell differentiation; skeletal development; cartilage condensation

Disease: Osteogenesis Imperfecta, Type Xiii