Typical Testing Data/Standard Curve (for reference only)
Chicken Aryl Hydrocarbon Receptor Nuclear Translocator 2 ELISA Kit | ARNT2 elisa kit
Chicken Aryl Hydrocarbon Receptor Nuclear Translocator 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ARNT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARNT2 antibody and an ARNT2-HRP conjugate. The assay sample and buffer are incubated together with ARNT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARNT2 concentration since ARNT2 from samples and ARNT2-HRP conjugate compete for the anti-ARNT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARNT2 from the sample, fewer sites are left to bind ARNT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARNT2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting additional roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively. [provided by RefSeq, Dec 2013]
Uniprot Description
ARNT2: Specifically recognizes the xenobiotic response element (XRE). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 15q24
Cellular Component: transcription factor complex; cytoplasm; nucleus
Molecular Function: signal transducer activity; DNA binding; aryl hydrocarbon receptor binding; protein heterodimerization activity; transcription factor activity
Biological Process: central nervous system development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; brain development; signal transduction; response to estradiol stimulus; negative regulation of apoptosis
Disease: Webb-dattani Syndrome
Research Articles on ARNT2
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Product Notes
The Chicken ARNT2 arnt2 (Catalog #AAA745602) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA745602 ELISA Kit recognizes Chicken ARNT2. It is sometimes possible for the material contained within the vial of "Aryl Hydrocarbon Receptor Nuclear Translocator 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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