OTUB2 recombinant protein

OTUB2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121312

• Gene Names: OTUB2; OTB2; OTU2; C14orf137
• Purity: >97% as determined by SDS-PAGE
• Synonyms: OTUB2; OTUB2 Protein; Human; Recombinant (His Tag); Human OTUB2 Protein (His Tag); C14orf137 Protein; OTB2 Protein; OTU2 Protein; OTU deubiquitinase; ubiquitin aldehyde binding 2; OTUB2 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His234

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human OTUB2 (Q96DC9-1)(Met 1-His 234) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for OTUB2 recombinant protein

Background: Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded beta-sheet sandwiched in between a small helical amino-terminal region consisting of alpha1 and alpha2, and a large helical region comprised of alpha3-alpha10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains.

References

Balakirev MY, et al. (2003) Otubains: a new family of cysteine proteases in the ubiquitin pathway. EMBO Rep 4 (5): 517-22.Nanao MH. (2004) Crystal structure of human otubain 2. EMBO reports. 5: 783-8.

NCBI and Uniprot Product Information

• NCBI GI #: 12962939
• NCBI GeneID: 78990
• NCBI Accession #: NP_075601.1
• NCBI GenBank Nucleotide #: NM_023112.3
• UniProt Accession #: Q96DC9; Q6IA10; Q9H6T1
• Molecular Weight: 8,637 Da
• NCBI Official Full Name: ubiquitin thioesterase OTUB2
• NCBI Official Synonym Full Names: OTU deubiquitinase, ubiquitin aldehyde binding 2
• NCBI Official Symbol: OTUB2
• NCBI Official Synonym Symbols: OTB2; OTU2; C14orf137
• NCBI Protein Information: ubiquitin thioesterase OTUB2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; deubiquitinating enzyme OTUB2; otubain-2; ubiquitin-specific protease otubain 2; ubiquitin-specific-processing protease OTU
• UniProt Protein Name: Ubiquitin thioesterase OTUB2
• Protein Family: Ubiquitin thioesterase
• UniProt Gene Name: OTUB2
• UniProt Synonym Gene Names: C14orf137; OTB2; OTU2
• UniProt Entry Name: OTUB2_HUMAN

NCBI Description

This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]

Uniprot Description

OTUB2: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C65 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Hydrolase; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 14q32.12

Cellular Component: nucleus

Molecular Function: ubiquitin-specific protease activity

Biological Process: protein deubiquitination; proteolysis