Transferrin Active Protein | TF active protein

Transferrin Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123002

• Gene Names: Trf; HP; Tf; Tfn; hpx; Cd176; AI266983
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Transferrin; Transferrin Protein; Mouse; Recombinant (hFc Tag); Mouse Transferrin/Serotransferrin Protein (Fc Tag); Cf-3 Protein; Cf3 Protein; F3 Protein; transferrin; TF active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His697

• Species: Mouse
• Activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 5-40ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 20
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse TRF (Q921I1)(Met1-His697) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 5-40 ng/mL.)

Related Product Information for TF active protein

Background: Transferrin is a glycoprotein with an approximate molecular weight of 76.5kDa. This glycoprotein is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of Transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. Transferrins are iron binding transport proteins that bind Fe³⁺ ion in association with the binding of an anion, usually bicarbonate. This transferrin binds only one Fe³⁺ ion per protein molecule. Transports iron ions from the hemolymph into the eggs during the vitellogenic stage. Transferrins are iron binding transport proteins which can bind two Fe(³⁺) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin loaded with iron encounters with a transferring receptor on cell surface, transferring binds to it and, as a consequence, is transported into the cell in a visicle by receptor-mediated endocytosis. The PH is reduced by hydrogen iron pumps. The lower pH causes transferrin to release its iron ions. The receptor is then transported through the endocytic cycle back to the cell surface, ready for another round of iron uptake. Each transferrin molecule has the ability to carry two iron ions in the ferric form.

References

Ponka P, et al. (1998) Function and regulation of transferrin and ferritin. Semin Hematol. 35(1): 35-54.Wagner E, et al. (1990) Transferrin-polycation conjugates as carriers for DNA uptake into cells. Proc Natl Acad Sci. 87(9): 3410-4.Cheng Y, et al. (2004) Structure of the human transferrin receptor-transferrin complex. Cell. 116 (4): 565-76. Karey, K.P. et al. (1988) Cancer Research 48:4083.

NCBI and Uniprot Product Information

• NCBI GI #: 20330802
• NCBI GeneID: 22041
• NCBI Accession #: NP_598738.1
• NCBI GenBank Nucleotide #: NM_133977.2
• UniProt Accession #: Q921I1; O35421; Q3UBW7; Q58E69; Q61803; Q62357; Q62358; Q62359; Q63915; Q64515; Q8VII5; Q922C0
• Molecular Weight: 76,724 Da
• NCBI Official Full Name: serotransferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: Trf
• NCBI Official Synonym Symbols: HP; Tf; Tfn; hpx; Cd176; AI266983
• NCBI Protein Information: serotransferrin
• UniProt Protein Name: Serotransferrin
• Protein Family: Transferrin
• UniProt Gene Name: Tf
• UniProt Synonym Gene Names: Trf; Transferrin

Uniprot Description

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.