Human Myocardin ELISA Kit | MYOCD elisa kit

Human Myocardin ELISA Kit

Cat. Num. AAA764395

• Gene Names: MYOCD; MYCD
• Reactivity: Human
• Synonyms: Myocardin; Human Myocardin ELISA Kit; MYOCD/MYCD (Myocardin)/BSAC2A/MYCD/MYOCD/Srfcp/; MYOCD elisa kit

• Reactivity: Human
• Sequence Length: 986

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10nmol/ml
• Sensitivity: 0.094nmol/L
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for MYOCD elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human Myocardin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Human Myocardin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Myocardin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Myocardin can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 54306542
• NCBI GeneID: 93649
• Molecular Weight: 106,769 Da
• NCBI Official Full Name: myocardin
• NCBI Official Synonym Full Names: myocardin
• NCBI Official Symbol: MYOCD
• NCBI Official Synonym Symbols: MYCD
• NCBI Protein Information: myocardin
• UniProt Protein Name: Myocardin
• Protein Family: Myocardin
• UniProt Gene Name: MYOCD
• UniProt Synonym Gene Names: MYCD
• UniProt Entry Name: MYCD_HUMAN

NCBI Description

This gene encodes a nuclear protein, which is expressed in heart, aorta, and in smooth muscle cell-containing tissues. It functions as a transcriptional co-activator of serum response factor (SRF) and modulates expression of cardiac and smooth muscle-specific SRF-target genes, and thus may play a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

myocardin: Smooth muscle cells (SM) and cardiac muscle cells- specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleus

Molecular Function: histone acetyltransferase binding; histone deacetylase binding; protein binding; transcription factor binding

Biological Process: alveolus development; cardiac cell differentiation; cardiac muscle cell differentiation; gut development; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of smooth muscle cell differentiation; positive regulation of smooth muscle contraction; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of cell growth by extracellular stimulus; regulation of histone acetylation; regulation of myoblast differentiation; regulation of smooth muscle cell differentiation; response to hypoxia; smooth muscle cell differentiation; transcription from RNA polymerase II promoter; urinary bladder development; uterus development; vasculogenesis; ventricular cardiac muscle cell differentiation