OLFM4 / GW112 recombinant protein

Recombinant Human OLFM4 / GW112 Protein (His tag)

Cat. Num. AAA2545710

• Gene Names: OLFM4; GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: OLFM4 / GW112; Recombinant Human OLFM4 / GW112 Protein (His tag); OLFM4; GC1; GW112; KIAA4294; OLM4; OlfD; UNQ362; bA209J19.1; hGC-1; hOLfD; OLFM4 / GW112 recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 510

• Application Notes: The recombinant human OLFM4 consists of 501 amino acids and predictes a molecular mass of 56.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh OLFM4 is approximately 65-70 kDa due to different glycosylation.
• Predicted N Terminal: Asp 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for OLFM4 / GW112 recombinant protein

Background: Olfactomedin-4, also known as G-CSF-stimulated clone 1 protein, Antiapoptotic protein GW112, and OLFM4, is a secreted protein which contains one olfactomedin-like domain. The OLFM4 gene was recently reported to inhibit various apoptotic pathways and promote proliferation of cancer cells, suggesting that OLFM4 might serve as a marker for human cancers. Thus, OLFM4 mRNA might be a useful tool to support the identification of cancer, irrespective of the clinical stages. It is overexpressed in a number of human tumor type s, especially in those of the digestive system. GW112 is associated with GRIM-19, a protein known to be involved in regulating cellular apoptosis. Functionally, GW112 could significantly attenuate the ability of GRIM19 to mediate retinoic acid-IFN-beta-mediated cellular apoptosis and apoptosis-related gene expression. In addition, GW112 demonstrated strong antiapoptotic effects in tumor cells treated with other stress exposures such as hydrogen peroxide. Finally, forced overexpression of GW112 in murine prostate tumor cells led to more rapid tumor formation in a syngeneic host. OLFM4 is an important regulator of cell death that plays important roles in tumor cell survival and tumor growth. As a candidate gene for cancer-specific expression. The serum olfactomedin 4 (OLFM4) is a useful marker for Gastric cancer (GC) and its measurement alone or in combination with Reg IV has utility in the early detection of GC. GW112 has an antiapoptotic property against the cytotoxic agents-induced apoptosis. It suggested that GW112 could be an important mediator in NF kappaB-dependent tumorigenesis of digestive tract tissues.

Description: A DNA sequence encoding the human OLFM4 (NP_006409.3) (Met 1-Gln 510) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 32313593
• NCBI GeneID: 10562
• NCBI Accession #: NP_006409.3
• NCBI GenBank Nucleotide #: NM_006418.4
• UniProt Accession #: Q6UX06; O95362; Q5VWG0; Q86T22
• Molecular Weight: 57,280 Da
• NCBI Official Full Name: olfactomedin-4
• NCBI Official Synonym Full Names: olfactomedin 4
• NCBI Official Symbol: OLFM4
• NCBI Official Synonym Symbols: GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1
• NCBI Protein Information: olfactomedin-4
• UniProt Protein Name: Olfactomedin-4
• UniProt Gene Name: OLFM4
• UniProt Synonym Gene Names: GW112; OLM4; hGC-1

NCBI Description

This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. [provided by RefSeq, Mar 2011]

Uniprot Description

OLFM4: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: azurophil granule; extracellular space; perinuclear region of cytoplasm; plasma membrane; secretory granule; specific granule

Molecular Function: cadherin binding; protein binding; protein homodimerization activity

Biological Process: protein homooligomerization; regulation of apoptosis; regulation of phagocytosis