Mouse 2'-5'-oligoadenylate synthase 1A ELISA Kit | Oas1a elisa kit

Mouse 2'-5'-oligoadenylate synthase 1A ELISA Kit

Cat. Num. AAA288142

• Gene Names: Oas1a; L3
• Reactivity: Mouse
• Synonyms: 2'-5'-oligoadenylate synthase 1A; Mouse 2'-5'-oligoadenylate synthase 1A ELISA Kit; Oas1a elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse (2-5')oligo(A) synthase 1A. No significant cross-reactivity or interference was observed.
• Sequence Length: 358

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/ml
• Sensitivity: 0.159 ng/mL
• Intra-Assay CV: <=4.6%
• Inter-Assay CV: <=7.1%
• Recovery: 92%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Oas1a elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to (2-5')oligo(A) synthase 1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for (2-5')oligo(A) synthase 1A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain (2-5')oligo(A) synthase 1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of (2-5')oligo(A) synthase 1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 281332108
• NCBI GeneID: 246730
• NCBI Accession #: NP_660212.2
• NCBI GenBank Nucleotide #: NM_145211.2
• UniProt Accession #: P11928; Q3UEC1; Q64440; Q91VI0; F2Z419
• Molecular Weight: 42,429 Da
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase 1A
• NCBI Official Synonym Full Names: 2'-5' oligoadenylate synthetase 1A
• NCBI Official Symbol: Oas1a
• NCBI Official Synonym Symbols: L3
• NCBI Protein Information: 2'-5'-oligoadenylate synthase 1A
• UniProt Protein Name: 2'-5'-oligoadenylate synthase 1A
• Protein Family: 2'-5'-oligoadenylate synthase
• UniProt Gene Name: Oas1a
• UniProt Synonym Gene Names: Oias1; (2-5')oligo(A) synthase 1A; 2-5A synthase 1A
• UniProt Entry Name: OAS1A_MOUSE

Uniprot Description

OAS1: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L. Belongs to the 2-5A synthase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.84; Transferase

Cellular Component: cytoplasm; nucleus

Molecular Function: 2'-5'-oligoadenylate synthetase activity; double-stranded RNA binding; protein binding; zinc ion binding

Biological Process: negative regulation of viral reproduction