Typical Testing Data/Standard Curve (for reference only)
Mouse 5-hydroxytryptamine receptor 1B ELISA Kit | Htr1b elisa kit
Mouse 5-hydroxytryptamine receptor 1B ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-HT1B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 5-HT1B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 5-HT1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 5-HT1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
5-HT(1B): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1
Cellular Component: cytoplasm; integral to membrane; integral to plasma membrane; membrane; plasma membrane
Molecular Function: drug binding; G-protein coupled receptor activity; serotonin binding; serotonin receptor activity; signal transducer activity
Biological Process: behavior; bone remodeling; drinking behavior; feeding behavior; G-protein coupled receptor internalization; G-protein coupled receptor protein signaling pathway; negative regulation of cAMP biosynthetic process; negative regulation of serotonin secretion; negative regulation of synaptic transmission, GABAergic; negative regulation of synaptic transmission, glutamatergic; protein kinase C activation; regulation of behavior; regulation of dopamine secretion; response to cocaine; response to drug; response to ethanol; response to toxin; serotonin receptor, adenylate cyclase inhibiting pathway; signal transduction; synaptic transmission; vasoconstriction
Research Articles on Htr1b
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Product Notes
The Mouse Htr1b htr1b (Catalog #AAA289883) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA289883 ELISA Kit recognizes Mouse Htr1b. It is sometimes possible for the material contained within the vial of "5-hydroxytryptamine receptor 1B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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